相关实验视频

Updated: Jul 1, 2026

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

The Mouse Round-window Approach for Ototoxic Agent Delivery: A Rapid and Reliable Technique for Inducing Cochlear Cell Degeneration

Published on: November 26, 2015

通过半圆通道给予D-银糖会诱导耳加速衰老:一种新型的氧化应激介导的前列腺瘤模型

Chunli Zhao¹, Zijing Yang¹, Zhongrui Chen¹

¹Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Clinical Center for Hearing Loss, Capital Medical University, Beijing 100050, China.

|December 21, 2025

概括

研究人员开发了一种加速耳衰老的新模型,以研究与年龄相关的听力损失. 这种有针对性的方法在小鼠中迅速诱导听力损伤和听觉系统退化.

科学领域:

耳鼻喉科耳鼻喉科耳鼻喉科
衰老研究研究衰老研究
神经科学是一个神经科学.

背景情况:

与年龄相关的听力损失 (ARHL) 是一种常见的感觉神经损伤,具有重大后果.
现有的ARHL模型存在局限性,包括长时间和高可变性.
需要一个更有效,更可靠的模型来研究耳衰老.

研究的目的:

开发和验证一种新的,加速的耳衰老模型.
将衰老病理集中在外周听力系统内.
为研究ARHL机制提供一个工具.

主要方法:

通过后半圆通道开发了一个有针对性的D-银糖 (D-gal) 输送模型.
利用听觉脑干反应测试来确定最佳的D-gal度和持续时间.
进行了形态染色,免疫组织化学和线粒体膜潜能评估.

主要成果:

在第14天,D-gal-H组表现出与ARHL一致的听觉特征.
观察到显著的外皮毛细胞损失和丝带突触退化.
检测到高氧化应激标志物 (4-HNE,8-OHdG) 和改变的衰老蛋白,以及线粒体去极化.

结论:

建立了一个快速,有针对性和病理验证的耳衰老模型.
该模型有效地复制了ARHL在外围听觉系统中的关键特征.
该模型是研究与年龄相关的听力损失机制的宝贵工具.

关键词:

这是ARHLHL.D-银糖是什么? D-银糖是什么?氧化应激是一种氧化应激.一个半圆形的运河.

更多相关视频

Cochlear Surface Preparation in the Adult Mouse

Cochlear Surface Preparation in the Adult Mouse

Published on: November 6, 2019

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

相关概念视频

The Cochlea

The Cochlea

The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.