鉴定TaqW-6AS,一种控制小麦谷物WE-AX含量的新基因
Qiong Li1,2,3, Zhankui Zeng1,2, Yue Zhao1,2
1College of Agronomy, Henan University of Science and Technology, Luoyang, 471000, Henan, China.
概括
在小麦染色体6AS上发现了一种名为TaqW-6AS的新基因,该基因会影响水中可提取的阿拉比诺xylan (WE-AX) 含量. 这一发现有助于通过标记器辅助的选择来改善小麦的生物强化和健康益处.
科学领域:
- 植物遗传学和育种方法
- 分子生物学分子生物学
- 营养科学 营养科学
背景情况:
- 可在水中提取的阿拉比诺基兰 (WE-AX) 是小麦谷物中重要的可溶性食纤维,具有显著的健康益处和功能性质.
- 识别控制WE-AX含量的遗传因素对于通过育种改善小麦营养质量至关重要.
研究的目的:
- 为了确定与小麦WE-AX含量相关的定量特征位点 (QTLs).
- 克隆和描述负责WE-AX内容调节的基因.
- 在小麦育种计划中开发用于标记器辅助选择 (MAS) 的分子工具.
主要方法:
- 基因组广泛关联研究 (GWAS) 和链接分析在两个不同的小麦种群 (ZZ和AH) 上进行.
- 用Axiom小麦90K SNP阵列和多样性阵列技术 (DArT) 进行基因型鉴定.
- 在已识别的QTL区域内克隆了一个候选基因,并研究了其蛋白质定位.
主要成果:
- 在多个环境中,在6A染色体的短臂上始终检测到一个具有重大影响和稳定的QTL,QWE-AX.haust-6A.
- 在QWE-AX.haust-6A.候选区域内发现了一种新型基因,被指定为TaqW-6AS,编码III类血红素过氧化酶,被确定为QWE-AX.haust-6A.候选区域.
- 发现TaqW-6AS蛋白位于ER-Golgi分泌通路内.
结论:
- 已识别的TaqW-6AS基因在调节小麦谷物的WE-AX含量方面发挥着重要作用.
- 三个功能标记物和TaqW-6AS的两个有利的单元类型成功开发和验证了标记物辅助选择.
- 这项研究为增强WE-AX含量和通过育种改善小麦的营养价值提供了宝贵的遗传资源.
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