跨元动物的FoxQ2转录因子的进化动态揭示了三种古老的类似物
Giacomo Gattoni1,2, Che-Yi Lin3, Joshua R York4
1Department of Zoology, University of Cambridge, Cambridge, UK. gg2900@columbia.edu.
Communications biology
|December 21, 2025
概括
福克斯Q2基因家族的起源很古老,在双边国家中发现了三种类似基因. 这些同类物质的重复和次功能可能推动了动物中FoxQ2的快速进化.
科学领域:
- 进化发育生物学 进化发育生物学
- 基因组学就是基因组学.
- 进行比较基因组学.
背景情况:
- 福克斯Q2是一种保存的转录因子,具有不清楚的进化史和脊椎动物本地化.
- 之前的研究显示了快速分歧,血统特异性基因损失和FoxQ2.2的重复.
- 相互矛盾的报道需要对FoxQ2进化的全面分析.
研究的目的:
- 为了澄清动物类中的FoxQ2基因的进化历史和谱.
- 识别和描述古老的FoxQ2对应物及其表达模式.
- 了解驱动FoxQ2进化的调节机制.
主要方法:
- 来自21个动物族群的FoxQ2序列的遗传学和合成分析.
- 对来自不同物种的单细胞转录基因数据集的分析.
- 在多个脊椎动物和无脊椎动物模型中进行光 in situ 杂交.
主要成果:
- 在双边关系中发现了三种古老的FoxQ2对应物 (FoxQ2I,FoxQ2II,FoxQ2III).
- 在脊椎动物中保留了两个FoxQ2对应物,这表明它们的谱系比以前知道的更丰富.
- 福克斯Q2I和福克斯Q2II表现出保守的前部表达,而福克斯Q2III则在状肠内皮表达.
- 保存的转录因子结合部位揭示了FoxQ2I的细胞类型特定的调节相互作用.
结论:
- 早期FoxQ2I/II对应物的重复促进了亚功能化,这有助于FoxQ2的快速进化速度.
- 福克斯Q2III代表着一个独特的,内皮表达在状动物内paralog.
- 这项研究为FoxQ2基因家族提供了一个全面的进化框架.
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