在麦 (Fagopyrum spp.) 中的维生素B概况和维生素GWAS. ):第一份报告
Madhiya Manzoor1, Ammarah Hami1, Jebi Sudan1
1Present Address: Proteomics Laboratory, Division of Plant Biotechnology, Sher-E-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu and Kashmir India.
概括
用LC-MS/MS分析了麦种子面粉的九种必需的B族维生素. 全基因组关联研究确定了维生素含量的QTL,有助于改善营养的繁殖.
科学领域:
- 农业科学 农业科学
- 营养科学 营养科学
- 基因组学就是基因组学.
背景情况:
- 维生素是从外部来源获得的关键微量营养素.
- 麦是一种营养丰富的伪谷物,具有显著的健康益处.
- 维生素B缺乏会导致各种健康问题,包括贫血和出生缺陷.
研究的目的:
- 建立一个关于在麦中进行维生素B分析的协议.
- 分析不同麦基因型中的九种必需的B族维生素.
- 通过全基因组关联研究 (GWAS) 识别与维生素积累相关的定量特征位置 (QTL) 和候选基因.
主要方法:
- 开发了一种液体染色学-并联质谱学 (LC-MS/MS) 协议,用于B族维生素分析.
- 分析了来自116种不同麦基因型的种子面粉,检测了胺 (B1), рибофлавин (B2), 尼亚 (B3), 尼古丁胺 (B3), 潘托酸 (B5), 铁素 (B6), 伊诺西托尔 (B8), 叶酸 (B9), 和 科巴胺 (B12).
- 在 4,142,684 个已识别的变体 (SNP 和 InDels) 上进行了全基因组关联研究 (GWAS),以绘制维生素含量 QTL 的地图.
主要成果:
- 在不同麦基因型中观察到维生素B含量的显著变化.
- 识别和映射了与分析的九种B族维生素的积累相关的QTL.
- 这项研究代表了麦中维生素-GWAS的第一个报告.
结论:
- 鉴定到的基因组洞察力可以为育种计划提供信息,以提高麦的营养质量.
- 现代分析和基因组方法对于优化作物改进策略至关重要.
- 这项研究为开发含有更高维生素B含量的麦品种提供了基础,以应对全球营养挑战.
相关概念视频
02:21Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
01:11Genome-wide Association Studies-GWAS
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...