益生菌B240激活谷氨酸-ROS通路,增加了巨细胞对流感的反应
1Department of Respiratory and Critical Care Medicine, Tianjin Chest Hospital, Tianjin, China.
Frontiers in cellular and infection microbiology
|December 22, 2025
概括
口服的Lactobacillus pentosus B240通过增强谷氨反应性氧物种 (GSH-ROS) 缓冲和巨类型I干扰素 (IFN) 反应来增强对H1N1的先天免疫力. 这种益生菌加强了早期的抗病毒防御,这表明它有可能用于呼吸道病毒感染预防.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 计算生物学 计算生物学
背景情况:
- 流感诱导的肺损伤结果取决于巨细胞I型干扰素 (IFN) 轴的激活.
- 这种激活需要严格调节的谷氨-反应性氧物种 (GSH-ROS) 缓冲.
研究的目的:
- 为了确定口服*Lactobacillus pentosus* B240是否会放大巨-IFN反应.
- 调查B240是否预调节GSH-ROS通路以增强对H1N1病毒的先天免疫力.
主要方法:
- 使用ComBat批次校正,limma,GSVA,WGCNA和CIBERSORTx对GSE43764公共数据集进行了重新分析.
- 统计分析包括多次比较的Benjamini-Hochberg调整.
主要成果:
- 在未感染的小鼠中,B240上调的肺Gclc和基线GSH-ROS/Nrf2得分.
- 后H1N1挑战,B240放大GSH-ROS,Nrf2,巨细胞激活,以及I型IFN得分.
- 基因表达分析揭示了B240治疗小鼠中的巨标记物和IFN刺激基因 (ISG) 的上调;WGCNA确定了一个将GSH-ROS和巨丰度联系起来的模块;CIBERSORTx证实了巨透率的增加和M1/M2指数.
结论:
- B240提高了基线GSH-ROS值,并增强了巨细胞透和I型IFN反.
- 这加强了对H1N1感染的早期天生的免疫反应.
- 建议*Lactobacillus pentosus* B240作为高风险呼吸道病毒暴露的营养免疫预防剂.
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