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敲除Y盒结合蛋白2诱导线粒体功能障碍,以中断猪胚胎中雄性基因组激活

Wen-Jie Jiang^1,2, Song-Hee Lee², Hyeon-Ji Song²

¹College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.

Journal of animal science and technology

|December 22, 2025

概括

Y盒结合蛋白2 (YBX2) 对于早期猪胚胎发育至关重要. 淘汰YBX2会损害线粒体功能,致虫基因组激活和组蛋白修饰,阻碍胚胎的进展.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

Y盒结合蛋白2 (YBX2) 是一种涉及mRNA调节的生殖细胞特异蛋白.
它在早期猪胚胎发育中的确切作用尚不清楚.

研究的目的:

研究YBX2在早期猪胚胎发育中的功能.
阐明YBX2影响胚胎进展的分子机制.

主要方法:

在单细胞阶段的猪胚胎中使用siRNA微注射诱导YBX2淘汰 (KD).
使用定量实时聚合酶连锁反应 (qRT-PCR),西部涂抹和免疫光染色.
评估了胚胎细胞的发育率,线粒体活动,细胞体基因组激活 (ZGA) 和基因组修饰 (H3K9ac,H3K9me3).

主要成果:

YBX2 KD显著降低了胚胎细胞率和线粒体活动.
YBX2 KD 降低了线粒体生物发生因子 (NRF1,NRF2,SIRT1) 的表达和线粒体功能受损.
YBX2 KD 干扰了母体到胚胎的过渡,增加了母体mRNA水平,同时降低了ZGA mRNA水平和母体蛋白质水平.
YBX2 KD改变了基因组修饰,减少了H3K9ac和增加了H3K9me3,表明对基因转录的影响.

结论:

YBX2对于早期的猪胚胎发育至关重要.
YBX2通过影响线粒体生物发生和胚胎基因组激活来调节胚胎发育.
YBX2在表观遗传调节中发挥作用,在早期胚胎发生过程中影响基因基因修饰和基因转录.

关键词:

线粒体中的线粒体.猪胚胎的使用情况在Y盒结合蛋白2中.紫毒的基因组激活

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