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自然和重组过敏性花生蛋白在人工胃肠道液体中的消化能力

Mizuho Terashima¹, Rina Matsuoka¹, Takumi Nishiuchi^2,3,4

¹Graduate School of Horticulture, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

Food safety (Tokyo, Japan)

|December 22, 2025

概括

与天然花生过敏原 Ara h 1 和 Ara h 2 相比,重组蛋白质上的多胺标签 (His-tags) 并没有显著改变消化能力. 这支持在对转基因食品的安全评估中使用它们.

科学领域:

食品安全和生物技术
对转基因生物 (GMO) 的过敏性评估.

背景情况:

对转基因食品的安全评估需要评估新表达蛋白质 (NEP) 的过敏性潜力.
素和素等蛋白质酶的消化性是评估过敏原潜力的关键标准.
通常用于蛋白质净化的多胺标签 (His-tags) 对蛋白质消化的影响尚不清楚.

研究的目的:

为了比较天然花生过敏原 (Ara h 1,Ara h 2) 与含有His标签的重组对应物的消化能力.
研究His标签对蛋白质对素和素消化敏感性的影响.
用液体染色学-并联质谱法 (LC-MS/MS) 分析蛋白质分解裂痕部位.

主要方法:

使用天然和重组的Ara h 1和Ara h 2蛋白质上的素,素和胰腺素进行消化性测试.
重组蛋白质的蛋白质分解与N-终端 (Ara h 1) 和C-终端 (Ara h 2) 的标签.
用LC-MS/MS分析片段,以确定裂变部位.

主要成果:

他的标签在C端延伸处显示了素消化中的微小差异,但没有影响其他裂解部位.
在Ara h 1和Ara h 2的N端区域的皮层对自然和重组形式的素消化具有同等的抗性.
一种较短的,可能错折的,重组的Ara h 2异型表现出由于可能的二硫化键形成而降低了素的敏感性.

结论:

具有His标签的重组蛋白质在很大程度上显示出与天然过敏原可比的蛋白质酶消化能力.
标记His的重组蛋白可以可靠地用于对转基因食品的安全性评估.
这项研究确定了一种更短的重组Ara h 2异型,具有改变的消化能力,突显了特征重组蛋白质变体的重要性.

关键词:

一个小时的时间.时间 h 2 2 时间过敏原蛋白质是一种过敏原蛋白质.消化性测试测试可以消化.新表达的蛋白质.花生花生花生花生花生

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