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Updated: Jan 8, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

转内肝移植系统分流患者的循环微生物组概况:16S rRNA与猎枪测序对比

Jingxiang Zhang¹, Xiaoshuang Xu¹, Lei Chen¹

¹Hebei Key Laboratory of Gastroenterology, Department of Gastroenterology, Hebei Clinical Research Center for Digestive Diseases, Shijiazhuang, Hebei Institute of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei, China.

Frontiers in medicine

|December 22, 2025

概括

16S rRNA测序检测到血液中的微生物信号比猎枪元基因组学更为多样化. 该研究发现,在血管区之间存在稳定和均的微生物社区结构,这表明血液微生物组的组成一致.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

鉴定循环微生物群的特征是具有挑战性的,因为血液样本处理缺乏标准化的协议.
现有的血液微生物社区分析方法需要进一步验证和比较.

研究的目的:

为了比较16S rRNA amplicon测序和shotgun metagenomic测序在检测循环微生物群中的有效性.
评估血液循环系统内的微生物社区结构和多样性.

主要方法:

从10名接受跨内肝移植系统 (TIPS) 程序的患者中采集了无菌血液样本.
使用基于ASV的方法与USEARCH和VSEARCH进行16SrRNA (V3-V4区域) 的分析.
使用Kraken2-Bracken管道的猎枪元基因组数据的分类学分类.
系统地比较两种测序方法生成的数据.

主要成果:

16S rRNA测序产生了一个更广泛的微生物信号的范围,相比枪元基因组学.
在两种方法的分类学概况中观察到有限的重叠,但确定了核心微生物群.
保存的核心微生物群落在血管区间表现出稳定的α和β多样性指数.

结论:

16S rRNA amplicon 测序在捕获循环微生物群中的多样化微生物信号方面比 shotgun metagenomics 更有效.
在整个循环系统中存在稳定和均的微生物社区结构,无论血管区分如何.

关键词:

16S rRNA 是一个16S rRNA.提示提示 (TIPS) 是一个提醒.流通中的微生物群.门肝循环 - 门肝循环猎枪测序的顺序是什么

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