相关实验视频
Updated: Jun 12, 2026

08:51
Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
14.0K
蛋白质对碳纳米点细胞吸收的影响
Ziyao Liu1, Huijie Yan1, I Jénnifer Gómez2,3
1Fachbereich Physik, Universität Hamburg, ChyN, Luruper Chaussee 149, Hamburg 22607, Germany.
ACS omega
|December 22, 2025
概括
用人血清白蛋白 (HSA) 预涂层碳纳米颗粒 (CD) 增强了细胞吸收,而血清暴露减少了它. 这项研究阐明了蛋白质冠冕对纳米粒子内部化的影响.
科学领域:
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
背景情况:
- 蛋白质冠状体显著影响纳米粒子-细胞相互作用.
- 了解蛋白质冠状形成动态对于纳米医学应用至关重要.
- 预制与现场蛋白质冠状形成对细胞吸收的影响仍未得到充分研究.
研究的目的:
- 为了研究预制蛋白质冠状和现场冠状形成对碳纳米点 (CD) 细胞吸收的影响.
- 为了比较CD的细胞内化与预制的人类血清白蛋白 (HSA) 冠状病毒,与那些暴露于血清或裸体CD的细胞内化.
主要方法:
- 碳纳米颗粒 (CD) 经过人血清白蛋白 (HSA) 的预化.
- 细胞吸收量使用流细胞计和共聚焦激光扫描显微镜量化.
- 实验是在无血清和含血清条件下进行的.
主要成果:
- 血清补充条件导致CD细胞吸收的显著减少.
- 带有HSA的CD的预化增强了它们被细胞内部化.
- 在预形成的HSA冠状病毒和血清形成的冠状病毒之间观察到吸收的明显差异.
结论:
- 在纳米颗粒上形成蛋白质冠状,特别是它的时间和组成,极大地调节了细胞吸收.
- 预涂层纳米粒子与特定的蛋白质,如HSA可以是一个策略,以提高目标细胞的交付.
- 血清成分可能会干扰或改变纳米粒子-蛋白相互作用,导致吸收减少.
相关概念视频
Electron Carriers
Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Protein Transport into the Inner Mitochondrial Membrane
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
Transport of mitochondrial precursors across the TIM23 channel is driven by...
Protein Import into the Peroxisomes
Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Protein Transport to the Inner Chloroplast Membrane
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

