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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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相关实验视频

Updated: Jan 8, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
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iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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snoCLASH揭示了广泛的snoRNA-mRNA交互网络

Brittany A Elliott1, Gene Yang1, Alex K Choi1

  • 1Department of Medicine, Duke University Medical Center, Durham, NC, 27705, USA.

bioRxiv : the preprint server for biology
|December 22, 2025
PubMed
概括
此摘要是机器生成的。

小核RNAs (snoRNAs) 指导RNA的修改. 一种名为snoCLASH的新方法揭示了成千上万种非核糖体RNA相互作用,揭示了snoRNA在基因调节中的新角色.

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 基因组学就是基因组学.

背景情况:

  • 小核RNAs (snoRNAs) 主要指导核糖体RNA (rRNA) 的修饰.
  • 新出现的证据表明,snoRNA与非rRNA目标相互作用,但由于rRNA的丰富性和技术障碍,发现是具有挑战性的.
  • 对这些非正规相互作用的系统识别对于理解更广泛的RNA调节至关重要.

研究的目的:

  • 开发和验证一种可扩展的方法来识别转录组范围内的snoRNA-non-rRNA相互作用.
  • 发现snoRNAs的新型mRNA目标及其相关的调节功能.
  • 为探索超越rRNA修饰的非正规snoRNA功能建立一个平台.

主要方法:

  • 开发snoCLASH,一种基于RNA结合蛋白 (RBP) 的优化交联,结合和测序框架.
  • 技术的整合包括-醇提取,聚A丰富,核分离,rRNA枯竭和双参考化学读取分析.
  • 应用snoCLASH用于转录组规模识别snoRNA-mRNA相互作用.

主要成果:

  • 识别数千个与snoRNA相关的mRNA区域,包括编码和调控元素.
  • 在与snoRNA相关的区域中,与表体转录组调节相关的已识别的RBP的丰富.
  • 功能验证snoRNA-mRNA相互作用,证明snoRNA依赖的2'-O-甲基化影响蛋白质表达.

结论:

  • snoCLASH是一个可扩展的平台,用于在转录组中发现和验证非正规的snoRNA目标.
  • 这种方法扩展了snoRNAs已知的功能,超出了rRNA修饰的范围.
  • 这些发现突出了snoRNAs在通过mRNA相互作用和表观转录组修饰来调节基因表达中的重要作用.