相关实验视频

Updated: Jan 8, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Dot1L授权DNA脱甲基化,以建立监管性T细胞身份

James J Cameron, Tyler R Colson, Xudong Li

bioRxiv : the preprint server for biology

|December 22, 2025

概括

调节性T (Treg) 细胞需要DNA脱甲基化才能发挥作用. 在这个过程中,Dot1L介导的H3K79甲基化是必不可少的,它招募TET酶并保持Treg的身份,以预防自身免疫性疾病.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

Foxp3⁺ 调节性T (Treg) 细胞对于免疫抑制和保持自我耐受性至关重要.
建立和维持Treg身份依赖于特定基因的DNA脱甲基化.
驱动Treg特异性DNA脱甲基化的精确表观遗传机制仍然不完全理解.

研究的目的:

阐明基因组修饰在Treg特异性DNA脱甲基化中的作用.
确定参与Treg细胞身份和功能的关键表观遗传调节剂.

主要方法:

研究了在Treg细胞中Dot1L催化基因素H3素79甲基化 (H3K79me) 的作用.
利用Treg特异性基因删除模型和针对TET酶的药理干预.
在操纵的Treg细胞中评估了DNA脱甲基化,基因表达和免疫功能.

主要成果:

通过Dot1L介导的H3K79me对于Treg特异性DNA脱甲基化在胸膜和诱导Treg血统中至关重要.
H3K79me将TET家族DNA脱甲基酶招募到关键的调节位点,促进染色体激活.
破坏Dot1L会导致DNA脱甲基化受损,Treg基因表达减少,以及致命的自身免疫性疾病.

结论:

确定了一个关键的表观遗传轴:Dot1L介导的H3K79甲基化驱动了TET依赖的DNA脱甲基化.
这个轴对于维护Treg细胞的身份,功能和免疫平衡至关重要.
药理上增强TET活性可以在Dot1L抑制的情况下挽救Treg功能.

更多相关视频

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...