相关实验视频

Updated: May 8, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

充电扫描突变生成作为一个高通量方法用于表位图映射的表位图映射

Kawkab Kanjo¹, Munmun Bhasin¹, Chandrani Dey^1,2

¹Molecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru, India.

Protein science : a publication of the Protein Society

|December 22, 2025

概括

这项研究引入了一种使用条码充电扫描突变发生的快速表位图谱绘制技术. 这种方法加快了病毒相互作用部位的识别,有助于疫苗和抑制剂的开发.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

识别中和表位是开发有效疫苗和抗病毒抑制剂的关键.
充电残留物替代物 (例如,酸,氨酸) 在暴露的抗原位置上耐受性良好,对蛋白质稳定性影响最小.
这种对表位残留的替代可以显著破坏与同类伴侣的结合.

研究的目的:

开发和验证一种使用条形码带电扫描突变发生的快速表位映射方法.
为了识别针对SARS-CoV-2免疫原体的多克隆血清所准的中和表位.
通过改进相互作用位点识别来加速疫苗和抑制剂的开发.

主要方法:

酵母表面显示带条码的充电扫描突变发生的图书馆.
使用流细胞计结合深度测序进行查.
为SARS-CoV-2受体结合域构建一个Aspartate扫描库,每个突变都与独特的条形码相关联.

主要成果:

开发的方法成功地绘制了针对SARS-CoV-2免疫原体免疫的小鼠的表位.
带有条码的充电扫描突变发生利用图书馆的多样性>50倍低于完整的突变扫描.
这种方法促进了图书馆的建设,选,下游分析和样本的多重化.

结论:

这种带有条形码的充电扫描突变发生方法为表位图绘制提供了更快,更有效的方法.
该技术加速了对病毒抗原的关键相互作用部位的识别.
这一进步具有显著的潜力,可以加速针对病毒病原体的疫苗和抑制剂的开发.

关键词:

充电扫描扫描的使用.标本地图绘制标本地图绘制图书馆图书馆图书馆是一个突变发生的突变发生.受体结合领域是受体结合领域.

更多相关视频

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

相关概念视频

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Continuous Charge Distributions

Continuous Charge Distributions

Imagine a bucket of water. It contains many molecules, of the order of 1026 molecules. Thus, although it contains discrete elements (molecules) at the microscopic level, macroscopically, it can be considered continuous. Small volume elements of water, infinitesimal compared to the bulk of the bucket's volume, still contain many molecules. Under this framework, quantized matter is approximated as continuous for practical purposes.
The electric charge can also be subjected to an analogical...