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通过原始生殖细胞移植在肉中产生生殖基因基梅拉的逐步程序

Hee Jung Choi¹, Yujin Han¹, Jae Yong Han²

¹Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University.

Journal of visualized experiments : JoVE

|December 22, 2025

概括

这项研究提出了一种可靠的协议,用于使用原始生殖细胞 (PGC) 生成生殖基因组. 这种方法增强了鸟类转基因,基因组编辑和保护工作.

科学领域:

鸟类生物技术鸟类生物技术
发育生物学是发展生物学.
遗传学是一个遗传学.

背景情况:

传统的鸟类转基因方法在生殖系传播效率方面存在局限性.
原始生殖细胞 (PGC) 移植提供了一种更有效的策略,因为它们具有固有的生殖细胞能力.

研究的目的:

建立一个分阶段的协议来隔离,培养和移植的PGCs.
为了研究和保护,可靠地生成生殖线嵌合体.

主要方法:

从HH阶段26-28胚胎中分离PGCs.
在体外维护和扩展PGCs.
将PGC移植到HH14-17期受体胚胎的背部大动脉中.
使用光标记物 (PKH26) 监测移植,并通过PCR确认生殖系的贡献.

主要成果:

持续地生成可行的生殖基因线嵌合体.
捐赠者PGCs在受体生殖腺中的成功集成和功能能力.
与基因组编辑工具 (如CRISPR/Cas9和转子子向量) 的兼容性.

结论:

开发的协议为鸟类生殖系操纵提供了一个强大的框架.
这种方法促进了转基因和基因编辑的鸟类模型的生产.
该战略支持通过跨物种生殖系传播和遗传保护来保护鸟类.

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