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内巨RIL-seq发现了沙门氏菌病毒性相关小RNAPINT的RNA对手

Hoda Kooshapour^1,2, Gianluca Matera², Elisa Venturini²

¹Department of Microbiology, Biocenter, University of Würzburg, Würzburg D-97074, Germany.

Nucleic acids research

|December 22, 2025

概括

沙门氏菌的毒性依赖于小型调节性RNA (sRNA). 一项新的研究显示,InvS通过作为诱来对抗Pint,一种毒性调节剂,从而提供了对沙门氏菌细胞内感染控制的见解.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌学细菌学是一门学科.

背景情况:

沙门氏菌的毒性取决于SPI-1和SPI-2的病原性岛屿.
像Pint这样的Hfq依赖的小调节RNA (sRNA) 在转录后控制病毒性基因表达.
在宿主细胞入侵期间,Pint调节了SPI-1和SPI-2表达之间的切换.

研究的目的:

为了研究在沙门氏菌的细胞内感染期间Pint活动的反调节.
在巨细胞内绘制沙门氏菌的RNA互动组图,以确定新的调节相互作用.
阐明PinT介导的抑制缓解的机制.

主要方法:

优化了RIL-seq以映射感染巨细胞内的沙门氏菌中的RNA-RNA相互作用.
生物化学测试以确认RNA相互作用和调节功能.
遗传实验验证已识别的sRNA在病毒性基因调节中的作用.

主要成果:

在细胞内感染期间,RIL-seq为Hfq介导的相互作用提供了全面的视图.
3'端衍生的sRNA InvS被确定为Pint的直接负调节器.
InvS充当诱,缓解PinT介导的抑制,并抑制MipA mRNA;PinT与InvS的相互作用缓解了这种抑制.

结论:

在沙门氏菌中发现了一对对抗性sRNAs,Pint和InvS.
InvS使用诱机制来抵消Pint的监管活动.
这些发现揭示了在沙门氏菌毒性调节中进行转录后控制的新层.

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