通过逆向工程设计I型毒素-抗毒素系统进行转录后调节的正交和便携式RNA装置的设计
Guobin Yin1,2, Luyao Zhang1,2, Di Zhou1,2
1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Nucleic acids research
|December 22, 2025
概括
研究人员将I型毒素-抗毒素 (TA) 系统重新用于合成RNA装置,称为SRTS-OPRTS,用于基因调节. 这些便携式设备可以实现动态遗传电路和对所需突变物进行选择性丰富.
科学领域:
- 合成生物学 合成生物学
- 分子生物学分子生物学
- 微生物遗传学微生物遗传学
背景情况:
- I型毒素-抗毒素 (TA) 系统是调节基因表达的原生生物遗传元素.
- 它们的正规功能包括通过RNA介导的翻译抑制来维护等离子体.
- TA系统具有固有的模块化和快速动力学,这表明有可能重新定位.
研究的目的:
- 将I型TA系统逆向工程化为便携式转录后调节RNA装置.
- 开发一个平台,用于生成对称的合成调节性毒素-抗毒素 (SRTS-OPRTS) 对,适用于不同细菌物种.
- 为了证明这些合成RNA设备在构建动态遗传电路和选择性丰富系统中的实用性.
主要方法:
- 类型I TA对的核心组件的隔离和表征.
- 人工TA对 (SRTS-OPRTS) 的重建,具有定义的结构和能量约束.
- 开发一种跨物种平台,用于生成正交的SRTS-OPRTS对.
- 应用SRTS-OPRTS作为表达元件和3' UTR标签用于定量基因调节.
- 构建相互抑制开关和用于突变丰富的选择性致命系统.
主要成果:
- 在TA系统结构和镇压功能之间展示了独立性.
- 成功生成了对称的SRTS-OPRTS对,适用于细菌细菌,大肠杆菌和Corynebacterium glutamicum.
- 使用SRTS作为表达元件或3' UTR标签实现了定量基因调节.
- 构建了动态的相互抑制的遗传开关.
- 开发了一种选择性致命系统,可将平均光强度提高11.32倍.
结论:
- 重新设计的I型TA系统作为基因调节的多功能合成RNA装置.
- 开发的SRTS-OPRTS平台提供了便携式和直角工具,用于构建动态遗传电路.
- 这些合成RNA装置为先进的基因工程应用提供了坚实的基础,包括选择性突变丰富.
相关概念视频
Types of RNA
72.4K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.4K
Types of RNA
8.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
8.9K
Translational Regulation
503
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
503
Transcriptional Regulation: Riboswitches
514
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
514
Experimental RNAi
7.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
RNA Interference
27.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.7K


