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经过妊娠期pyrifos暴露后,神经元活动和血清体受体表达的持续增加

Jeffrey A Koenig¹, Nathan Cramer¹, Kara Kessler¹

¹Department of Neurobiology and UM-MIND University of Maryland School of Medicine, Baltimore, MD 21201.

|December 22, 2025

概括

孕期暴露于pyrifos (CPF) 杀虫剂增加了大鼠大脑中自发的神经元的激发. 这种神经毒性可能是神经发育障碍的基础,例如自闭症谱系障碍.

科学领域:

神经科学是一个神经科学.
发育神经科学的发展神经科学.
环境毒理学环境毒理学

背景情况:

围产期暴露于pyrifos (CPF) 与神经发育障碍有关.
以前的研究表明,CPF暴露后发生了突触传输和血清素受体结合的改变.
功能性大脑变化在很大程度上是未知的.

研究的目的:

调查孕期CPF暴露是否会增加抑制性内部神经元的自发发射.
确定CPF暴露是否会改变发育中的大脑中的血清素受体表达.

主要方法:

用一个大鼠模型来对妊娠期CPF暴露.
采用ex vivo电生理学来评估体感皮层中神经元的激发.
利用免疫组织化学和RNAscope in situ杂交来分析神经元活动和受体表达.

主要成果:

暴露于CPF的后代在体感皮层中增加了自发的神经元发射.
快速升的帕瓦胺 (PV) 内神经元被确定为过活神经元的一个子集.
在男性后代的PV神经元中观察到5-HT1B受体的表达增加.

结论:

孕期CPF暴露可能导致持续的体感皮质过度兴奋.
这些神经生理学变化可能会导致CPF相关的行为缺陷.
这些发现提供了有关神经发育障碍的机制的见解,这些障碍与早年暴露于杀虫剂有关.

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