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[关于昼夜节律扰乱对斑马鱼免疫功能影响的单细胞转录组分析]

L Liu¹, Q Guo¹, A Li¹

¹Department of Pediatrics, The Third Xiangya Hospital of Central South University, Changsha 410013, China.

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]

|December 22, 2025

概括

循环节律的破坏改变了斑马鱼的免疫细胞,增加了激活的T细胞,改变了免疫细胞的发育. 这揭示了单细胞洞察力,即睡眠中断如何影响免疫力.

科学领域:

免疫学免疫学免疫学
时间生物学时间生物学
发展生物学发展生物学

背景情况:

已知循环节律扰乱会影响免疫功能,但潜在的单细胞机制尚不清楚.
了解这些机制对于解决与睡眠障碍有关的健康问题至关重要.

研究的目的:

用斑马鱼模型调查昼夜节律扰乱对免疫细胞结构和功能在单细胞水平的影响.
识别受改变光暗周期影响的特定免疫细胞亚群及其发育轨迹.

主要方法:

斑马鱼幼虫被暴露在恒定的光线 (24小时LL) 中,以破坏昼夜节律,对照组在14小时的光/10小时的黑暗 (14:10小时LD) 周期中.
单细胞RNA测序被用来对免疫细胞进行分析和分析.
用UMAP聚类,标记基因注释和Monocle伪性时代分析来描述细胞亚型和发育路径.

主要成果:

确定了七种免疫细胞亚型,包括T细胞,巨细胞,中性粒细胞和NK细胞.
循环节律的破坏导致激活的T细胞显著增加 (18.11%与7.61%) 与免疫激活标志物的表达增加.
伪物质分析表明淋巴细胞的分布和成熟发生了变化,尽管核心发育轨迹完好无损.

结论:

循环节律的破坏导致斑马鱼免疫系统的显著结构和功能重塑.
观察到T和NK细胞的增强激活和免疫反应状态的转变.
这些发现提供了关键的单细胞分辨率洞察力,了解中断的昼夜节律和睡眠障碍的免疫后果.

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