相关实验视频
Updated: Jan 8, 2026
10:06
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
8.2K
单细胞转录组测序揭示了肝细胞异质性,以应对辐射诱导的肝损伤和再生
Liqing Li1, Qiaoyuan Wu1, Zhitao Lin1
1Department of Radiation Oncology, Guangxi Medical University Cancer Hospital, Nanning, China.
概括
辐射诱导性肝损伤 (RILI) 涉及不同的肝细胞修复程序. 2区肝细胞重新填补受损区域,而3区肝细胞显示脆弱性,指导RILI的新治疗点.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 辐射瘤学 辐射瘤学
- 分子生物学分子生物学
背景情况:
- 辐射诱导性肝损伤 (RILI) 限制了肝癌的放射治疗.
- RILI和随后的肝脏再生的机制尚未完全理解.
研究的目的:
- 阐明肝细胞对RILI的反应的时空动态.
- 为了确定特定的肝细胞亚群和信号通路,参与肝脏在辐射后的修复.
主要方法:
- 集成的单细胞和单核RNA测序在部分肝脏辐射的老鼠模型中.
- 人类肝脏组织的蛋白质分析.
- 轨迹分析以确定细胞重新定居的动态.
主要成果:
- 肝细胞表现出空间分工:增殖和凝固的第2区域,细胞循环抑制和代谢适应的1/3区域 (p53,PPARα-YAP1-TEAD1).
- 2区的肝细胞是主要的再生细胞,而1区的肝细胞首先再生.
- 周中心 (区域3) 肝细胞是脆弱的,显示高和S相停止.
- 观察到区域间交叉 (VEGFA,HGF) 和保存的人类通路激活 (p53,ERK,PPAR/YAP).
结论:
- 提出了一种辐射损伤后肝脏再生的空间模型.
- 空间上不同的肝细胞子群利用了分隔的修复程序.
- 研究结果表明,潜在的治疗目标可以缓解RILI.
更多相关视频
08:15A Hepatocellular Cancer Patient-Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth
Published on: February 2, 2024
1.4K
08:52Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
Published on: February 18, 2017
11.6K
相关概念视频
01:24Liver Regeneration
4.2K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.2K
01:58Cell Specific Gene Expression
16.2K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.2K