经历铁亡的M2巨细胞调节子宫光滑肌的收缩,以促进分娩开始
Limin Song1,2, Yue Wu1, Kunpeng Liu1
1Department of Gynecology and Obstetrics, the Fourth Affiliated Hospital of Harbin Medical University, No. 37 Yiyuan Street, Nangang District, Harbin, 150001, Harbin, China.
Scientific reports
|December 22, 2025
概括
细胞死亡过程铁亡,通过影响免疫细胞和子宫肌肉功能,在分娩开始中发挥关键作用. 这项研究确定了与ferroptosis相关的基因和参与启动分娩的途径.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 细胞死亡机制 细胞死亡机制
背景情况:
- 劳动开始机制复杂且不完全理解.
- 孕产妇的免疫反应是分娩开始的关键驱动因素.
- 铁,一种依赖于铁的细胞死亡,是一种潜在的免疫调节器,其在分娩中的作用尚未被探索.
研究的目的:
- 为了调查在分娩开始时发生的铁亡.
- 为了确定铁灭菌如何影响免疫细胞和子宫细胞功能.
- 确定与ferroptosis相关的基因和参与分娩启动的途径.
主要方法:
- 分析多队列转录组数据集以确定免疫细胞透 (CIBERSORT,ssGSEA).
- 拉索回归用于识别核心免疫细胞 (例如M2巨细胞).
- 不同基因表达分析,KEGG/GO丰富,ROC建模,斯皮尔曼相关性,体外/体内实验.
主要成果:
- 确定了49个与铁生相关的差异基因 (FRDGs) 和丰富的铁生/铁恒常通路.
- 证实了 ferroptosis 参与子宫,具有高的 Fe2+,降低了 T-GSH,并在分娩期间增加了 MDA.
- 铁灭症诱导的前炎性M2巨细胞和受损的人类子宫光滑肌细胞 (HUSMC) 功能.
结论:
- 铁亡与分娩开始期间免疫微环境的变化有关.
- 铁亡会影响M2巨细胞表型和子宫光滑肌细胞收缩性.
- 与ferroptosis相关的基因代表了潜在的生物标志物和分娩时间异常的治疗标.
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