豆蛋白-素双蛋白复合体的pH循环诱导形成:相互作用机制,结构,功能和生物活性分子保护
Wenjing Dong1, Xiuxiu Teng1, Shiyu Lin1
1College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, P. R. China.
Journal of agricultural and food chemistry
|December 23, 2025
概括
这项研究使用豆蛋白 (CP) 和素 (CA) 创建了一种双蛋白复合体,以改善黄素 (Cur) 的功能. 新的pH循环方法增强了黄素.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 营养生物化学 营养生物化学
背景情况:
- 单个蛋白质具有功能限制.
- 结合植物和动物蛋白质提供了一个可持续的解决方案.
- 为黄素等生物活性化合物开发有效的输送系统至关重要.
研究的目的:
- 为了创建一个双蛋白复合物 (pH循环诱导的豆蛋白-素,pH-CP/CA) 以提高黄素负载和功能.
- 为了研究pH-CP/CA复合物的形成机制和特性.
- 为了评估封装效率,稳定性和生物可用性,在复合体内装载的黄素 (pH-CP/CA-Cur).
主要方法:
- 使用pH循环与小蛋白 (CP) 和素 (CA) 在2:1质量比下制造双蛋白复合物.
- 在pH-CP/CA.中对粒子大小,泽塔潜力和相互作用力 (键,静电,疏水) 的表征.
- 通过pH循环向pH-CP/CA复合物加载黄素 (Cur),形成pH-CP/CA-Cur.
- 对pH-CP/CA-Cur.的封装效率,稳定性和体外生物利用性的评估.
主要成果:
- 最佳的pH-CP/CA复合体 (CP/CA = 2:1) 显示了粒子大小的减少和增大的泽塔潜力.
- 复杂的形成是由结合,静电和疏水相互作用驱动的,提高了溶解度,乳化,泡和热稳定性.
- 与自由黄相比,pH-CP/CA-Cur实现了83.04%的封装效率,并证明了增强的稳定性和85.82%的生物可用性.
结论:
- pH循环是制造双蛋白复合物的有效方法.
- pH-CP/CA复合体作为一个有前途的纳米载体,可以增强黄素等生物活性分子的功能和生物可用性.
- 这种方法提供了一种可持续的策略,以克服食品和营养药物应用中单一蛋白质的局限性.
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