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基于UPLC-MS/MS的尿液中八种代谢物的检测方法的研究

Ling Sui¹, Qiuying Dong², Yudie Lu¹

¹School of Public Health, Hebei Medical University, Shijiazhuang 050017, PR China. Shihm@hebmu.edu.cn.

Analytical methods : advancing methods and applications

|December 23, 2025

概括

本研究提出了一种新的UPLC-MS/MS方法,用于检测尿液中的八种生物代谢物. 该方法准确地测量了暴露水平,有助于环境监测和职业健康评估.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

及其衍生物产生代谢物,在环境监测,职业健康和疾病诊断方面具有价值.
准确检测生物代谢物反映了真实暴露水平.

研究的目的:

开发和验证一种UPLC-MS/MS方法,用于确定人类尿液中的八种生物代谢物.
建立一个可靠的工具来评估生物样本中的暴露.

主要方法:

采用HLB列抽取尿液样本.
通过使用HSS T3液态染色学列的渐变化来实现分离.
检测是使用双重质谱法 (UPLC-MS/MS) 进行的.

主要成果:

该方法在所有八种分析物质中都显示出出色的线性 (相关系数> 0.999).
恢复率在80-120%之间,这表明它的准确性很高.
实现了低检测极限 (<0.011 ng mL-1),确保了灵敏度.

结论:

开发的UPLC-MS/MS方法简单,准确和精确.
该方法符合检测要求,适用于分析现实世界的尿样,以评估暴露.

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