相关实验视频

Updated: Jan 8, 2026

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"

Published on: March 31, 2021

用于传递CRISPR/Cas9系统的纳米载体

SuJin Hwang¹, Hyejun Ko¹, Hee-Young Lee^2,3

¹School of Integrative Engineering, Chung-Ang University, Seoul, Republic of Korea.

Nanomedicine (London, England)

|December 23, 2025

概括

克里斯普尔/卡斯9系统能够精确的基因组编辑,但面临着交付挑战. 本综述探讨了基于纳米载体的传递方法,以实现生物技术和医学中高效的CRISPR/Cas9应用.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

集群定期间隔的短巴林德罗姆重复/关联蛋白9 (CRISPR/Cas9) 系统是精确的DNA操纵和基因组编辑的强大工具.
克里斯普尔/Cas9技术对生物医学应用具有重大前景,包括疾病治疗和遗传查.

研究的目的:

审查和讨论CRISPR/Cas9系统的各种交付方法.
专注于基于纳米载体的技术,以实现高效和选择性的CRISPR/Cas9传递.
分析CRISPR/Cas9传递系统的挑战,未来方向和潜力.

主要方法:

使用科学网,Scopus,PubMed和谷歌学者进行全面的文献搜索.
对2015年至2024年间发表的文章进行分析.
专注于使用纳米载体的传递技术.

主要成果:

对CRISPR/Cas9系统的交付仍然是其广泛应用的关键障碍.
纳米载体是克服CRISPR/Cas9传递挑战的有希望的策略.
各种纳米载体系统为高效和选择性的CRISPR/Cas9传递提供了潜力.

结论:

有效和选择性交付对于释放CRISPR/Cas9技术的全部潜力至关重要.
纳米载体介导的传递系统是推动CRISPR/Cas9在生物技术和医学中的应用的关键.
对优化纳米载体传递的进一步研究对未来的治疗和研究进步具有重大前景.

关键词:

这就是CRISPR/Cas9的作用.基因编辑基因编辑基因治疗的基因疗法纳米载体纳米载体是什么纳米医药是一种纳米医药.

更多相关视频

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...