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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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LFC-plus:用于高通量单细胞分析的同时多色体积细胞计.

Zhi Ling1,2,3,4, Wenhao Liu1,2, Kyungduck Yoon1,2,3,4

  • 1Laboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA, USA. shu.jia@gatech.edu.

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概括

介绍LFC-plus,一种用于先进体积单细胞分析的新型光场细胞计平台. 这种高通量系统实现了亚细胞分辨率,使生物研究和诊断能够更深入地了解.

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科学领域:

  • 生物技术是生物技术.
  • 细胞生物学 细胞生物学
  • 光学工程是指光学工程.

背景情况:

  • 图像流细胞计面临着平衡分辨率,多重复合,吞吐量和复杂性的挑战.
  • 现有的平台往往难以同时提供体积和高分辨率单细胞分析.

研究的目的:

  • 推出LFC-plus,这是一个下一代光场细胞计测平台.
  • 为了实现多参数,同时多颜色和体积单细胞分析,提高分辨率和吞吐量.

主要方法:

  • 集成基于模型的图像恢复,定制光场光学和光谱光圈分区.
  • 开发一个实现3D亚细胞分辨率和大成像截面的平台.
  • 高通量数据采集,每秒近20万个单元.

主要成果:

  • LFC-plus在所有三个维度中都显示了亚细胞分辨率.
  • 该平台实现了近毫米尺度的成像截面.
  • 在各种应用中得到验证:化疗反应,细胞融合和迁移分析.

结论:

  • LFC-plus是一个强大的和可扩展的平台,用于高含量体积细胞计.
  • 该系统为基础生物学研究提供了重大进展.
  • 对转化诊断和高通量查有广泛的影响.