相关实验视频

Updated: Jan 8, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence

Published on: July 5, 2021

使用纳米粒子CuNCs作为过氧化酶模仿活动的酸酶的色度检测

Jinhua Fu¹, Gengneng Lai², Fang Ke²

¹Department of Pharmacy, Longyan First Hospital Affiliated to Fujian Medical University, Longyan, Fujian, China.

Luminescence : the journal of biological and chemical luminescence

|December 23, 2025

概括

铜纳米集群 (CuNCs) 提供一种低成本的纳米酶,用于检测酸酶 (ACP). 这种新的视觉传感器提供了敏感和特定的ACP量化,非常适合临床诊断.

科学领域:

生物化学生物化学
纳米技术纳米技术
分析化学分析化学

背景情况:

酸酸酶 (ACP) 是一种重要的疾病生物标志物.
传统的ACP检测方法往往是昂贵和复杂的.
铜纳米集群 (CuNCs) 具有类似于贵金属的催化性能,并且具有成本效益.

研究的目的:

开发一种可视色度传感器,用于使用CuNCs检测ACP.
为了利用CuNCs的过氧化酶模仿活性,用于ACP传感.
建立一个快速的,低成本的,简单的方法来量化ACP.

主要方法:

使用了具有内在过氧化酶类活性的CuNCs.
使用3,3',5,5'-四甲丁 (TMB) 作为基质.
开发了一种视觉色度测试法,其中ACP减少了CuNCs对TMB的氧化.

主要成果:

基于CuNCs的传感器对ACP具有很高的灵敏度和特异性.
实现了对ACP的0-6U/L的线性检测范围.
确定了ACP的0.027U/L的低检测极限.

结论:

开发的CuNCs平台为非洲国家和地区的检测提供了一个有前途的方法.
这种方法快速,经济高效,操作简单.
该传感器在临床诊断中具有很大的应用潜力.

关键词:

在此期间,阿克巴国家和地区的金机 CuNCs 金机颜色测量方法的颜色测量方法.过氧化酶的使用

更多相关视频

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Published on: June 6, 2022

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...