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在整个生态系统中发现聚酸盐和聚基酸盐累积生物:通过FACS测序进行表型向基因型定型

Yuan Yan¹, Mathew Baldwin¹, Jangho Lee^1,2

¹School of Civil and Environmental Engineering, Cornell University, Ithaca, New York 14850, United States.

Environmental science & technology

|December 23, 2025

概括

科学家们开发了一种新方法来发现储存多酸盐 (polyP) 和多酸酸盐 (PHA) 的微生物. 这一发现揭示了它们的广泛存在以及它们在植物生长和营养循环中的潜在作用.

科学领域:

微生物学微生物学
环境科学环境科学
生物技术是生物技术.

背景情况:

微生物生物聚合物,如多酸盐 (polyP) 和多基酸盐 (PHA) 对于适应至关重要.
聚酸盐积聚生物 (PAO) 通过代谢链接聚和PHA,从而实现增强的生物去除 (EBPR).
由于检测限制,EBPR以外的PHA-PAO的流行率和生态作用在很大程度上是未知的.

研究的目的:

开发和验证一种用于检测,量化和描述PHA-PAO的新方法.
调查环境样本中PHA-PAO的存在和多样性,包括EBPR系统和玉米根球.
探索新发现的PHA-PAO在促进植物生长和营养循环中的潜在功能.

主要方法:

开发了一种以表型为目标的方法,将三重染色光激活细胞分类与16S rRNA基因测序 (TriFlow-Seq) 结合起来.
使用光成像和单细胞拉曼光谱学验证了TriFlow-Seq.
将TriFlow-Seq应用于EBPR系统的活性污泥和玉米根球样本.

主要成果:

TriFlow-Seq成功地确定了已知的PAO (例如, *Candidatus* Phosphoribacter, *Tetrasphaera*, *Ca. * Accumulibacter) 和在罗多细菌中发现了新的PHA-PAO.
该方法揭示了各种PHA-PAO,包括玉米根球中的*Pseudomonas*,*Halomonas*和*Nannocystis*.
这些根系球PHA-PAO涉及到促进植物生长的活动,如酸盐溶解和植物激素生产.

结论:

TriFlow-Seq方法为研究PHA-PAO生态学提供了一个强大的工具.
PHA-PAOs在各种环境中普遍存在,包括农业土壤,并且在EBPR之外发挥作用.
由PHA-PAO产生的生物聚合物成本可能会显著影响树根球动力学和植物健康,其模式因植物基因型而异.

关键词:

环境的PAO是环境的PAO.以表型为目标的分类三重染色的FACS可以使用.

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