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神经细胞的招募和重编程作为胚胎肢体再生的核心驱动因素

Béryl Laplace-Builhé¹, Gautier Tejedor¹, Jholy De La Cruz¹

¹Institute for Regenerative Medicine and Biotherapies, University of Montpellier, INSERM, Montpellier 34295, France.

Proceedings of the National Academy of Sciences of the United States of America

|December 23, 2025

概括

哺乳动物的肢体再生是有限的,但早期的小鼠胚胎 (E10.5) 可以再生前肢芽. 这种能力依赖于由Wnt1和Foxd3标记物识别的神经衍生细胞 (NCdCs),这些细胞对这种再生过程至关重要.

科学领域:

发展生物学发展生物学
再生医学是一种再生医学.
哺乳动物胚胎发生

背景情况:

哺乳动物表现出有限的表形再生,与其他物种形成鲜明对比.
哺乳动物手指的远端部分是这种有限的再生能力的例外.
哺乳动物的早期胚胎阶段可能具有比以前理解的更大的再生潜力.

研究的目的:

为了研究早期哺乳动物肢体的再生能力.
确定前肢 (FB) 在小鼠胚胎中再生的细胞和分子机制.
确定特定细胞群在哺乳动物肢体再生中的作用.

主要方法:

在不同发育阶段的小鼠胚胎的比较转录组学 (E10.5与E12.5).
在体内谱系追踪,在FB发育和再生过程中追踪细胞群.
功能性研究涉及细胞枯竭和移植,以评估再生需求.

主要成果:

在E10.5的小鼠胚胎显示出启动FB再生的能力,这种能力在E12.5.5失去了.
确定了神经衍生细胞 (NCdCs) 的特定群体,在E10.5再生过程中重新表达早期标记物 (Wnt1,Foxd3).
这些NCdC对于FB再生至关重要,它们的缺失导致这种能力的丧失,这种能力可以通过移植恢复.

结论:

神经源细胞是哺乳动物前肢芽再生的关键先决条件.
早期哺乳动物胚胎中的短暂再生潜力是由特定的细胞群介导的.
这项研究提供了关于增强哺乳动物再生的潜力和要求的见解.

关键词:

一个小鼠胚胎.神经细胞是神经顶细胞.复兴再生是一种再生方式.

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