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相关概念视频

Gap Junctions01:27

Gap Junctions

9.3K
The cytoplasm of adjacent animal cells can exchange small molecules, ions, and secondary messengers via the communication channels which form the gap junctions. These junctions comprise a few hundred to thousands of molecular channels, each made of two halves, called the connexon hemichannel. A connexon is a hexamer of six transmembrane connexin proteins, which assemble radially, thus forming a pore or channel in the center. One connexon hemichannel docks with a corresponding connexon on the...
9.3K
Gap Junctions01:37

Gap Junctions

56.7K
Multicellular organisms employ a variety of ways for cells to communicate with each other. Gap junctions are specialized proteins that form pores between neighboring cells in animals, connecting the cytoplasm between the two, and allowing for the exchange of molecules and ions. They are found in a wide range of invertebrate and vertebrate species, mediate numerous functions including cell differentiation and development, and are associated with numerous human diseases, including cardiac and...
56.7K
Contact-dependent Signaling01:19

Contact-dependent Signaling

46.8K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
46.8K
Overview of Cell-Cell Junctions01:14

Overview of Cell-Cell Junctions

28.6K
The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another  cell. These cell junctions are classified  into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
28.6K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

8.8K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.8K
Biasing of P-N Junction01:16

Biasing of P-N Junction

1.7K
The operation of a p-n junction diode involves various biasing conditions, including forward bias, reverse bias, and equilibrium.
In equilibrium, no external voltage is applied across the p-n junction. The depletion region is formed at the junction interface due to the diffusion of carriers, which leaves behind charged dopants, acceptors on the p-side, and donors on the n-side. These immobile charges create an electric field that prevents further diffusion of carriers. The related energy band...
1.7K

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相关实验视频

Updated: Jan 7, 2026

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
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Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

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通过维生素B12和光线可控制的间隙连接.

Duo Cui1, Shuzhang Liu2, Xinyu Huang1

  • 1Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR, China.

Proceedings of the National Academy of Sciences of the United States of America
|December 23, 2025
PubMed
概括

研究人员开发了CarGAP,这是一种新的化学光遗传工具,用于精确控制间隙结. 该系统允许细胞间通信的可逆阻断和解锁,有助于理解生理过程.

关键词:
化学遗传学 化学遗传学差距交叉点的差距交叉点是一个问题.干细胞的利基是干细胞.

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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

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相关实验视频

Last Updated: Jan 7, 2026

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons
10:11

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

Published on: January 12, 2012

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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
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Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products

Published on: December 25, 2016

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 间隙连接对于细胞间通信和协调多细胞生物中的细胞活动至关重要.
  • 现有的控制间隙连接功能的方法缺乏精确的时空调节.

研究的目的:

  • 开发一种新的化学光遗传工具,CarGAP,用于精确和可逆地控制间隙结活动.
  • 通过使用CarGAP系统,研究干细胞-利基相互作用中差距结的作用.

主要方法:

  • 通过基因融合连接物与CarH_C的腺甲胺 (AdoB12) 结合域,产生脊椎动物的CarGAP (Cx-CarGAP).
  • 使用AdoB12诱导的蛋白质寡合化来阻断间隙结,并使用绿光诱导的拆解来重新打开它们.
  • 将CarGAP系统 (Inx-CarGAP) 应用于Drosophila卵巢,以控制由innexin2和innexin4形成的异型间隙结.

主要成果:

  • 在哺乳动物细胞中,Cx-CarGAP证明有效,可逆地阻断和恢复脊椎动物间隙连接通道.
  • Inx-CarGAP能够精确控制Drosophila卵巢异型间隙结,促进干细胞-利基相互作用的研究.
  • 卡尔加普系统被证明可以用于操纵脊椎动物和无脊椎动物系统中的间隙连接.

结论:

  • 卡尔加普 (CarGAP) 是一种多功能单组分化疗光遗传学工具,用于精确的时空控制间隙结.
  • 这种工具可以对由间隙结调节的多种生物功能进行探究.
  • CarGAP为在各种生物环境中研究细胞-细胞通信开辟了新的途径.