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Updated: Jun 29, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

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使用复制DNA单分子分析研究卡波西肉瘤相关性疹病毒的复制动力学的协议

Rajnish Kumar Singh1, Dipayan Bose1, Erle S Robertson1

  • 1Department of Otorhinolaryngology, University of Pennsylvania, Philadelphia, PA 19104, USA.

STAR protocols
|December 23, 2025
PubMed
概括

这项研究详细介绍了复制DNA单分子分析 (SMARD) 的协议,以调查卡波西的肉瘤相关的疹病毒复制动力学. 该方法可视化单个分子中的DNA合成,绘制复制起源和分叉进展的地图.

关键词:
癌症 癌症 癌症 癌症微生物学 微生物学分子生物学分子生物学

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Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...