细胞周期阶段,它们对细胞机械特性的影响,以及对候选细胞与宿主细胞相互作用的影响
Easter Ndlovu1, Zinnat Shahina1, Tanya E S Dahms1
1Department of Chemistry and Biochemistry University of Regina, 3737 Wascana Parkway, Regina S4S 0A2, SK, Canada.
ACS applied materials & interfaces
|December 23, 2025
概括
用原子力显微镜 (AFM) 测量细胞机制,揭示了健康和癌细胞之间的差异. 细胞周期阶段显著影响细胞弹性和病原体相互作用,对了解疾病至关重要.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 机械生物学 机械生物学
背景情况:
- 细胞机制,包括弹性和粘度,对于细胞分裂和迁移等生物过程至关重要.
- 了解细胞机制可以区分健康和患病细胞,有助于疾病进展研究.
- 诸如细胞形态,细胞骨重塑和细胞循环等因素影响细胞机械特性.
研究的目的:
- 使用原子力显微镜 (AFM) -微观生物学动态分析宿主细胞弹性和粘度.
- 研究细胞周期阶段对细胞机械性能的特定影响.
- 检查细胞周期依赖的机械变化对病原体与宿主相互作用的影响.
主要方法:
- 采用AFM-microrheology与合式探针进行动态机械分析,频率从1到200赫兹.
- 在不同细胞周期阶段 (G1,S,G2/M) 中利用化学同步的正常和癌细胞.
- 使用AFM进行单细胞力光谱,探测病原体与宿主相互作用 (Candida albicans与HeLa,HCT 116,HaCaT细胞).
主要成果:
- 与正常细胞 (92 Hz) 相比,癌细胞在较低频率 (48 和 63 Hz) 上表现出更粘性,类似流体的行为.
- 细胞弹性下降,损失触点增加,因为在细胞循环期间,actin细胞骨架被破坏.
- G1和S阶段的细胞比G2/M阶段的细胞具有较低的弹性模块 (较软);S阶段的细胞与Candida albicans的相互作用力增加.
结论:
- 细胞循环阶段显著改变细胞的机械性质,特别是弹性,这是由于actin细胞骨架重组.
- 在G1/S阶段的无组织的actin细胞骨导致弹性降低和对病原体相互作用的易感性增加.
- 细胞力学和细胞周期阶段是病原体与宿主相互作用的关键决定因素.
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