响应pH的释放水友性合物:氨酸/氨酸彩色纳米粒子及其水凝珠的制备和特征
Mengsi Yin1, Zijian Qiu1, Mengju Xu1
1College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Biomacromolecules
|December 23, 2025
概括
这项研究开发了一种新的纳米粒子-凝珠系统,以改善黄蛋白 (Lut) 和视网醇 (Ret) 的输送. 该系统增强了脂溶性生物活性物质的抗氧化特性和口服生物可用性.
科学领域:
- 营养科学 营养科学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 氨酸 (Lut) 和视网醇 (Ret) 具有协同的抗氧化功效,但在水溶性和稳定性方面面临挑战.
- 需要有效的输送系统来克服脂肪溶性生物活性物质的这些限制.
研究的目的:
- 开发一种"纳米粒子-凝珠"系统,用于同时提供黄蛋白和视网醇.
- 为了增强黄蛋白和视网醇的稳定性,抗氧化能力和肠道向性.
- 提高这些必需营养素的生物可访问性.
主要方法:
- 使用微流体技术与Zeen和PVP K30载体制备蛋白/视网醇共载纳米粒子 (L/R-NP).
- 将L/R-NP封装成藻酸盐-糖 (SA-Agar) 水凝珠 (L/RNP-GBs).
- 纳米粒子大小的表征,水凝珠的特性 (刚性,弹性),封装效率 (EE) 和pH响应的药物释放动力学.
- 使用DPPH试验和分子动力学模拟来评估自由基清除活动的结构稳定性.
主要成果:
- 成功制备了具有稳定的核心外结构的130nm L/R-NPs.
- 在SA-Agar水凝珠中实现了L/R-NP的高封装效率.
- 对于1:1的Lut:Ret比率,证明了卓越的抗氧化活性 (86.52%的自由基清除).
- L/RNP-GBs表现出pH响应的释放,pH值为1.2的释放最小,pH值为6.8的持续释放,表明肠道向.
- 水凝珠的配方提高了弹性,降低了吞障碍的风险.
结论:
- 纳米粒子-凝珠系统有效地克服了白蛋白和视网醇的输送挑战.
- 这种创新的交付策略增强了抗氧化作用和生物可访问性.
- 该系统显示出提供各种脂溶性生物活性物质的巨大潜力.
更多相关视频
09:11Alternating Magnetic Field-Responsive Hybrid Gelatin Microgels for Controlled Drug Release
Published on: February 13, 2016
10.2K
06:57Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
Published on: August 11, 2018
8.4K
相关概念视频
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
Water: A Bronsted-Lowry Acid and Base
The reaction between a Brønsted-Lowry acid and water is called acid ionization. For example, when hydrogen fluoride dissolves in water and ionizes, protons are transferred from hydrogen fluoride molecules to water molecules, yielding hydronium ions and fluoride ions:
pH Scale
Hydronium and hydroxide ions are present both in pure water and in all aqueous solutions, and their concentrations are inversely proportional as determined by the ion product of water (Kw). The concentrations of these ions in a solution are often critical determinants of the solution’s properties and the chemical behaviors of its other solutes. Two different solutions can differ in their hydronium or hydroxide ion concentrations by a million, billion, or even trillion times. A common means of...
pH
The potential of hydrogen (pH) is a measure of the acidity or basicity of a water-based solution determined by the concentration of hydronium ions (H3O+). In one liter of pure water at neutral pH, there are 1×10−7 moles of hydronium ions. However, the extensive range of hydronium ion concentrations present in water-based solutions makes measuring pH in moles cumbersome. Therefore, a pH scale was developed to convert moles of hydronium ions into the negative logarithm of the hydronium ion...
Basicity of Aliphatic Amines
Amines can behave as Brønsted–Lowry bases by accepting a proton from the acid to form corresponding conjugate acids. Due to a lone pair of nonbonding electrons, aliphatic amines can also act as Lewis bases by forming a covalent bond with an electrophile.
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
To measure the basicity of amines, two conventions are generally used. The first defines Kb as the basicity constant for the deprotonation reaction of water by the amine, as presented in Figure 1. Conventionally, lower Kb indicates higher...
Protein Buffers in Blood Plasma and Cells
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...
