单细胞RNA测序揭示了静止-衰老连续和化学疗法后不同的老型
Brianna Fernandez1,2, Victor J Passanisi1,2, Humza M Ashraf1,2
1Department of Biochemistry, University of Colorado, Boulder, CO, 80303, USA.
Nature communications
|December 23, 2025
概括
化疗会引起可逆静止和不可逆转衰老,两者难以区分. 这项研究揭示了它们存在于一个连续体上,由细胞循环动态和转录组形状联系在一起.
科学领域:
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
- 基因组学就是基因组学.
背景情况:
- 静止和衰老是癌症治疗中关键的细胞循环停止状态.
- 由于缺乏特定的生物标志物,很难区分这些状态.
- 这两种状态在化疗后同时出现,影响治疗结果.
研究的目的:
- 用先进的成像和单细胞RNA测序来区分静止和衰老.
- 将异质细胞周期表型与转录基因特征联系起来.
- 为了阐明导致细胞循环停止后化疗的途径.
主要方法:
- 将细胞周期动态的时间间隔成像与单细胞RNA测序结合起来.
- 使用埃托胺治疗来诱导细胞循环停止.
- 将伪时间轨迹分析应用于转录基因数据.
主要成果:
- 识别各种老化的类型 (老型).
- 描述两种截止途径:一种是渐进的途径,另一种是通过线粒体滑动的直接途径.
- 观察到老化的表型在早期和逐渐出现,甚至在浅处的静止细胞中.
结论:
- 静止和衰老存在于细胞循环退出连续在整个转录组的水平.
- 细胞周期动力学和转录基因特征是区分这些停止状态的关键.
- 了解这种连续性可以了解癌细胞逃避和治疗抵抗.
更多相关视频
相关概念视频
02:15Replicative Cell Senescence
4.2K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.2K
01:19Multipotency of Hematopoietic Stem Cells
3.8K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.8K