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开发和应用SNP标记物来区分韩国Perilla (Perilla frutescens) 品种,使用基因组序列变异

Jung-In Kim¹, Myoung Hee Lee¹, Sang Woo Kim¹

¹Upland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.

Scientific reports

|December 23, 2025

概括

研究人员开发了竞争性等位基特异性PCR (KASP) 标记器,以精确识别韩国Perilla品种. 这种具有成本效益的系统有助于品种认证,遗传纯度评估和Perilla frutescens的分子育种.

科学领域:

农业科学农业科学
基因组学就是基因组学.
植物育种植物育种

背景情况:

梨花果 (Perilla frutescens (L.) Britton) 是一种重要的拉米亚系物种,用于种子油和叶蔬菜.
准确识别和评估Perilla品种的遗传纯度对于育种和工业至关重要.

研究的目的:

开发和验证竞争性等位基特异性PCR (KASP) 标记物,以精确识别韩国Perilla品种.
建立最小的标记集,以区分种子类型和叶类型的Perilla.
评估KASP标记物对种子纯度评估的有用性.

主要方法:

对16种Perilla品种进行全基因组再测序,以确定单核酸多态 (SNP).
设计和验证使用48种品种的竞争性等位基特异性PCR (KASP) 标记物.
主坐标分析 (PCoA) 和植物遗传学分析用于品种歧视.
种子纯度评估的应用试验.

主要成果:

从全基因组再测序数据中识别了6183个高可靠性SNP.
在48个Perilla品种中验证了150个多态KASP标记.
建立最小的KASP标记集 (种类为5种,叶类为6种) 以区分韩国品种.
证明了KASP标记的有用性,用于快速,精确的品种识别和种子纯度评估.

结论:

开发的KASP标记系统为Perilla品种认证和遗传纯度评估提供了一种具有成本效益,高通量和可靠的方法.
这些基因组资源显著推进了Perilla分子育种计划和种子工业流程.
凯斯普系统有助于提高佩里拉遗传资源的管理和改进.

关键词:

佩里拉果 (Perilla frutescensens) 是一种鲜花的植物.种植物识别种植物识别遗传多样性遗传多样性这就是KASP标志的标志.种子纯度测试种子纯度测试

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