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单细胞转录组学揭示了静止的LEPR+子宫内膜介质干细胞中的隙调节

Yuan Fang^1,2, Dandan Cao², Cheuk-Lun Lee^1,3

¹Department of Obstetrics and Gynaecology, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, 999077, China.

Stem cell research & therapy

|December 24, 2025

概括

研究人员确定了一种表达LEPR.的原始,静止的人类子宫内膜干细胞 (eMSC) 亚群. 痕信号保持它们的茎状,为子宫内膜再生疗法提供了潜力.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
基因组学就是基因组学.

背景情况:

人体子宫内膜通过干细胞/祖细胞再生.
子宫内膜介质干细胞 (eMSCs) 经常通过CD140b和CD146标记物被识别出来.
更原始和静止的eMSC亚种群的身份尚未得到充分理解.

研究的目的:

识别和表征人类子宫内膜干细胞 (eMSCs) 中原始的,静止的亚种群.
研究Notch信号在维持eMSC干度和静止状态中的作用.

主要方法:

单细胞RNA测序 (scRNA-seq) 在培养的CD140b+CD146+eMSC上进行,并与现有的子宫内膜细胞数据集成.
一个LEPR+亚群被识别并使用体外测定,流动细胞计,免疫染和生物信息分析进行鉴定和特征化,包括细胞与细胞相互作用和伪实时轨迹推断.

主要成果:

在差异化轨迹的底部确定了一个独特的LEPR+eMSC亚群,表达了高水平的Notch受体.
这些LEPR+细胞表现出静止特征 (G0阶段) 和优越的自我更新和克隆性潜力,与LEPR-和批量eMSC相比.
发现隙信号组件,特别是JAG1和DLL1,对于维持LEPR+表型和静止来说至关重要.

结论:

LEPR+ eMSCs代表了人类子宫内膜干细胞的原始和静止子集.
缺口信号在保持这些LEPR+eMSC的干度和静止状态方面发挥着至关重要的作用.
这一发现对于推进子宫内膜再生策略具有显著的治疗潜力.

关键词:

这是一种克隆基因.子宫内膜再生子宫内膜再生胚胎内膜干细胞是什么?人类子宫内膜是人类子宫内膜.勒普丁受体的受体是什么?介质细胞干细胞介质细胞干细胞口信号传输是没有的.这是一个安静的安静.单细胞RNA测序的一个细胞.干细胞静止时间

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