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相关概念视频

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

5.4K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.4K
Ligand Binding Sites02:40

Ligand Binding Sites

14.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
14.8K
Conserved Binding Sites01:49

Conserved Binding Sites

5.0K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K
Riboswitches01:56

Riboswitches

9.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.5K

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相关实验视频

Updated: Jan 8, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
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A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

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通过对共价RNA向突变剖析进行位点选择性干选择.

Phillip Yesley1, Georgia Poulladofonou1, Danny Incarnato2

  • 1Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, Nijmegen, 6525 AJ, Netherlands.

Angewandte Chemie (International ed. in English)
|December 24, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了Covacil,一种新的RNA向的共价探头. 这种探测器可以选择性地修改FMN核糖开关,从而在复杂的生物样本中进行精确的RNA分析.

关键词:
协同变异的变化是共价变异.核酸化学中的核酸化学检测RNA的研究向RNA的RNA准方式在Riboswitch中使用.

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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相关实验视频

Last Updated: Jan 8, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
11:49

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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科学领域:

  • 化学生物学 化学生物学
  • 分子生物学分子生物学
  • 在RNA治疗方面,RNA疗法.

背景情况:

  • 向RNA的共价探头对于研究RNA结构和功能至关重要.
  • 设计针对特定RNA标的选择性探针仍然是一个重大挑战.
  • FMN рибо开关是细菌中一个关键的调节元件.

研究的目的:

  • 开发一种结构知情,自下而上的方法来设计向RNA的共价探头.
  • 为了创建一个高度基础选择性的共价探头,准FMN рибо开关.
  • 为了验证探头在复杂的RNA环境中的选择性和应用.

主要方法:

  • 利用突变分析和基于结构的设计策略.
  • 合成了一个基于Ribocil支架的共价探针库.
  • 对来自不同细菌物种的FMN рибо交换机体进行选探针.
  • 使用竞争性光亲和标记和质谱测量验证了探针选择性和协同性.

主要成果:

  • 鉴定了Covacil,这是一个高度基选择性的共价探头,用于FMN рибо开关的阿普坦.
  • 在10分钟内,Covacil在低微分子度下实现了共价变异.
  • 与非准探测器相比,证明了>1000倍的基础选择性.
  • 在总RNA样本中证实了Covacil对FMN рибо开关的选择性反应性.

结论:

  • 开发的结构知情方法使得能够设计出强大且有选择性的向RNA的共价探头.
  • 科瓦西尔是研究FMN рибо开关功能和RNA生物学的一个有价值的工具.
  • 科瓦西尔在总RNA中的基选择性反应性突显了其用于转录组分析的潜力.