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Updated: Jan 8, 2026

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从单细胞蛋白质量化数据中重建和比较信号传导网络

Tim Stohn1,2, Roderick van Eijl3, Klaas W Mulder3

  • 1Department of Computer Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.

Bioinformatics (Oxford, England)
|December 24, 2025
PubMed
概括

新的计算方法,单细胞模块响应分析 (scMRA) 和单细胞比较网络重建 (scCNR),使单细胞蛋白数据的信号传导网络分析成为可能. 这些方法揭示了特定于细胞群体的信号差异,以指导向癌症治疗.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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