De novo 蓝色的转录组数据集 Phlebodium aureum "Mandaianum" 这种类型的

相关实验视频

Updated: Jan 8, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Silviu Tomescu¹, Karl Rumbold^2,3, Selisha A Sooklal³

¹Analytic Panda (Pty) Ltd, Johannesburg, South Africa.

|December 24, 2025

概括

这项研究介绍了原性青Phlebodium aureum'Mandaianum'的第一个转录组,确定了用于酶生产和代谢途径探索的关键基因.

科学领域:

植物基因组学植物基因组学
生物学生物学
生物技术是生物技术.

背景情况:

黄金 (Phlebodium aureum) "曼迪安 (Mandaianum) "是一种蓝色的.
它产生一种 (R) 选择性氨酸酶 (HNL) 酶.
转录组对于理解其独特的生物化学是至关重要的.

研究的目的:

为了生成Phlebodium aureum"Mandaianum"的第一个全面的转录组数据集.
为了促进对异种表达的HNL基因的识别.
为了进一步研究二次代谢物生物合成和的代谢途径.

主要方法:

从叶片组织中提取总RNA.
使用Illumina HiSeq 2500的下一代测序.
在RNA-Seq的新组装中读到.
使用eggNOG Mapper (COG,GO,EC,KEGG,Pfam) 的生物信息注释.

主要成果:

产生了 76,562 个 de novo 组装的副本.
平均转录长度为954bp,N50长度为1427bp.
布斯科分析显示,76.5%的基因完全恢复.

结论:

生成的转录组为Phlebodium aureum'Mandaianum'提供了一个有价值的基因组资源.
它支持对HNL基因的识别和表征.
这一数据集将推进比较转录学,进化研究和的功能基因组学.

关键词:

生物催化剂是一种生物催化剂.蓝色原料的类.氨酸酸酸酸酶是什么?这就是Illumina Illumina.

更多相关视频

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Published on: May 12, 2023

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

相关概念视频

Non-vascular Seedless Plants

Non-vascular Seedless Plants

The diverse plant life on Earth—consisting of nearly 400,000 species—can be divided into three broad categories based on biological characteristics: nonvascular, seedless vascular, and seed plants.

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Seedless Vascular Plants

Seedless Vascular Plants

Seedless Vascular Plants Were the First Tall Plants on Earth