一种自然分离的共生菌Lactobacillus murinus通过微生物代谢物抑制了多药耐药的Campylobacter jejuni
Junhao Wang1,2, Ali Asif1,2, Fangtong Gu1,2
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
多种药物耐药的Campylobacter jejuni (MDR C. jejuni) 导致食物传播疾病. 牛乳杆菌 (Lactobacillus murinus) 通过分泌破坏其细胞膜的酸来抑制MDR C. jejuni,从而提供了一个潜在的益生菌战略.
科学领域:
- 微生物学 微生物学
- 肠道微生物组研究研究
- 食品安全 食品安全
背景情况:
- 耐多药Campylobacter jejuni (MDR C. jejuni) 是全球主要的食源性胃肠炎的原因之一.
- 肠道微生物群在防止MDR C. jejuni殖民方面发挥着作用,但机制尚未完全理解.
研究的目的:
- 通过多omics分析研究肠道微生物群在MDR C. jejuni殖民中的作用.
- 为了确定特定的肠道细菌及其代谢物抑制MDR C. jejuni.
主要方法:
- 在小鼠的肠道微生物群中进行了多个omics分析 (metagenomics, metabolomics).
- 在体外实验评估细菌抑制活性和机制.
- 从小鼠肠道菌群中分离和描述L. murinus.
主要成果:
- 泰尔瓦洛辛治疗改变了肠道微生物群,减少了Bifidobacterium longum和短链脂肪酸,这促进了C. jejuni的殖民.
- 确定了Lactobacillus murinus,并显示出对C. jejuni的体外抑制活性.
- L. murinus分泌的酸会诱导亡,破坏C. jejuni的细胞膜,并导致细胞溶解.
结论:
- 肠道微生物群失调可以促进C. jejuni的殖民.
- 牛乳杆菌 (Lactobacillus murinus) 通过酸分泌和细胞膜破坏,对C. jejuni具有抗菌性质.
- L. murinus代表了一种潜在的益生菌剂,可以控制C. jejuni感染.
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