相关实验视频

Updated: Jan 7, 2026

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Published on: October 28, 2013

细菌lux-生物传感器用于检测特定细胞对膜损伤的反应

Vladimir A Plyuta¹, Evgeny Y Gnuchikh^1,2, Anastasiia A Gorbunova³

¹Complex of NBICS Technologies, National Research Center "Kurchatov Institute", Kurchatov Sq. 2, 123182 Moscow, Russia.

|December 24, 2025

概括

新的全细胞生物传感器可以检测细菌膜损伤. 这些生物传感器使用PspA促销器和lux基因,在大肠杆菌和细菌细菌中表现出对各种膜损伤剂的特定应激反应.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

全细胞生物传感器是评估外部代理对细胞完整性的影响的宝贵工具.
来自大肠杆菌和细菌细菌的PspA应激诱导促进体提供了一种响应的遗传元件,用于检测细胞应激.

研究的目的:

构建和验证全细胞lux生物传感器,用于检测阴性 (大肠杆菌) 和阳性 (B. subtilis) 细菌中的膜损伤.
研究大肠杆菌和B. subtilis基于PSPA的生物传感器对各种膜损伤剂的差异反应.

主要方法:

通过将大肠杆菌和B. subtilis的PSPA促进体与Photorhabdus luminescens. lux基因融合,构建lux生物传感器.
用损坏膜的药物处理工程化大肠杆菌和B. subtilis,包括乙醇,Triton X-100,多素B,二甲基硫氧化物 (DMSO) 和梅利丁.
测量发光度以量化PSPA促进体诱导并评估细菌应激反应.

主要成果:

建造的lux生物传感器成功地检测到由乙醇,Triton X-100,polymyxin B,DMSO和melittin引起的膜损伤.
观察到不同的诱导模式:乙醇和polymyxin B在大肠杆菌和B. subtilis中都诱导了PSPA促进体,在大肠杆菌中幅度更高.
特里顿X-100和梅利丁在B. subtilis中特别诱导了PSPA促进体,而DMSO仅在大肠杆菌中诱导了它,突出了不同的应激反应.

结论:

开发的lux生物传感器是用于检测细菌膜损伤的功能性和高效的工具.
该研究表明,不同膜损伤剂对大肠杆菌和B. subtilis中的Psp应激反应系统的特定激活,揭示了特定物种的应激机制.

关键词:

B. subtilis 的意思,就是说.美国大肠杆菌 (E. coli).发光效应的发光效应膜损伤会导致膜损伤.膜透气化膜透气化 pspA pspA 的意思是pspA 全细胞生物传感器

更多相关视频

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

Published on: November 16, 2016

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens

Published on: July 8, 2011

相关概念视频

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...