基于树脂的牙科复合材料的细胞毒性和基因毒性,这些复合材料与四级盐进行了修改
Izabela Szymczak-Pajor1, Maja Zalega2, Joanna Nowak3
1Department of Nucleic Acid Biochemistry, Medical University of Lodz, 251 Pomorska Str., 92-213 Lodz, Poland.
Journal of functional biomaterials
|December 24, 2025
概括
乙三甲基化物 (CTAB) 在牙科复合材料中显示出显著的毒性,降低细胞活力并增加DNA损伤. 甲基二氧化十甲基化 (DODAB) 显示出卓越的生物相容性,使其成为下一代牙科材料的更安全选择.
科学领域:
- 生物材料科学 生物材料科学
- 牙科材料科学 牙科材料科学
- 毒理学 毒理学 毒理学
背景情况:
- 牙修复失败,通常是由于反复的,促使研究抗菌剂,如四级盐 (QASs).
- 含有QAS的树脂基复合材料 (RBC) 需要严格的生物相容性评估,因为与口腔组织接触.
研究的目的:
- 为了比较用 cetyltrimethylammonium 化物 (CTAB) 和 dimethyldioctadecylammonium 化物 (DODAB) 修饰的红细胞在体内生物相容性 (细胞毒性和遗传毒性).
- 评估CTAB和DODAB在被纳入实验性牙科复合材料时的安全性.
主要方法:
- 人体纤维细胞BJ细胞暴露在含有CTAB或DODAB的实验红细胞中的化物中.
- 用MTT测定来评估细胞活力.
- 细胞亡和亡通过Annexin V/Propidium Iodide (PI) 流动细胞计量得到量化.
- 用性彗星测定方法评估了DNA损伤.
主要成果:
- 在剂量取决的方式中,CTAB显著降低了BJ细胞活力,主要诱导晚期细胞亡/亡 (84%的降低在2重%的CTAB).
- 此外,CTAB还导致了剂量依赖的DNA损伤增加.
- 经DODAB修改的复合材料,以及对照材料 (KE,FA),表现出非细胞毒性和非基因毒性.
结论:
- 与CTAB相比,DODAB在合到牙科复合材料中显示出更高的细胞安全性.
- DODAB是开发安全的下一代抗菌牙科材料的有希望的候选人.
- 由于显著的毒性,CTAB在测试度下不适合临床牙科应用.
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