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挥发性芳香化合物,脂肪酸和LOX通路基因表达的比较分析两种Lentinula edodes的菌体

Changxia Yu¹, Jun Jiang², Mengke Zhang¹

¹National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

Journal of fungi (Basel, Switzerland)

|December 24, 2025

概括

一项关于Lentinula edodes mycelia的研究表明,石竹香气的差异是由于氧化酶 (LOX) 途径的变化. 菌株808具有较高的LOX活性,产生关键的芳香化合物,而突变型ww808具有较低的LOX活性和不同的代谢转化.

科学领域:

生物化学生物化学
菌类学菌类学是指菌类学.
代谢工程是代谢工程.

背景情况:

伦丁 (Lentinula edodes) 的香味主要研究在果实体中.
菌根香味的理解较少,但提供了对基本代谢途径的见解.
在L. edodes菌株808及其突变型ww808.8之间存在显著的芳香差异.

研究的目的:

阐明L. edodes mycelia在808菌株和突变型ww808菌株之间的芳香差异背后的机制.
确定关键的芳香生物标志物及其在L. edodes mycelia中的代谢起源.
为通过基因改造改善L. edodes香味质量提供理论基础.

主要方法:

气色谱-离子移动光谱法 (GC-IMS) 与主要成分分析 (PCA) 和正交部分最小平方差分分析 (OPLS-DA) 结合用于挥发性化合物分析.
脂肪酸组成,脂氧化酶 (LOX) 途径酶活性和基因表达水平的比较分析.
皮尔森相关性分析将芳香生物标志物与代谢因素联系起来.

主要成果:

808菌株的菌体表现出高的LOX基因表达和活性,积累了甲,甲和乙烯酸等芳香化合物.
突变的ww808,尽管C18:2前体较高,但显示出较低的LOX活性,限制了通路流动.
在ww808中,ADH2,ADH3和ADH5基因的表达增加和较高的酒精脱酶 (ADH) 活性导致了化转化,改变了芳香特征.

结论:

在L. edodes mycelia中的芳香差异主要是由LOX通路的基因表达和酶活性的改变驱动的.
LOX和ADH通路之间的平衡决定了特定挥发性化合物的积累,影响了整体香味.
这项研究提供了基础知识和潜在的目标,以有针对性地改善石竹香味.

关键词:

兰丁虫 (Lentinula edodes) 是一种有毒的植物.气味的差异,香气的差异.氧激酶 (LOX) 路径解释机制的解释机制细胞是什么意思?

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