优化的选择性介质增强了肠道衍生益生菌酵母的隔离和表征
Kevin Mok1,2, Kwantida Popitool1,2, Areerat Songla1
1Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Journal of fungi (Basel, Switzerland)
|December 24, 2025
概括
这项研究开发了一种文化学方法,以找到益生菌的肠道酵母. 几种安全的非Candida酵母菌株显示出有希望的抗微生物和应激生存能力,用于肠道健康应用.
科学领域:
- 微生物学 微生物学
- 益生菌研究 益生菌研究
- 肠道微生物组研究 研究肠道微生物组
背景情况:
- 与肠道相关的酵母因其作为益生菌的潜力而越来越受认可.
- 传统的隔离方法可能会受到细菌过度生长的阻碍.
- 鉴别酵母菌株的安全性和功能性对于益生菌的发展至关重要.
研究的目的:
- 应用指导式文化学工作流程来隔离和表征肠关联酵母作为益生菌候选人.
- 优化培养条件,从肠道微生物群中选择性分离酵母菌.
- 选隔离物安全性,耐压力和功能益生菌性质.
主要方法:
- 使用迪克森和修改的施德勒,使用选择性抗生素尾酒和在37°C的无氧化,优化了文化学.
- 从三个健康的捐赠者中分离出305个酵母菌株,随后排除了Candida和伪形态型.
- 进行安全查 (血液溶解,DNase,凝固酶),模拟胃肠道应力测试 (酸,胆),抗菌活性测试和胆盐酸酶活性评估.
主要成果:
- 成功分离了127种非Candida酵母菌株,其中26种在查后被认为是安全的.
- 在26个安全隔离物中,有20个在模拟的胃pH值为2.0或0.5%胆盐条件下显示出显著的生存率 (>50%).
- 几种分离物体表现出广泛的抗菌活性,对抗病原性细菌,如金黄色葡萄球菌,大肠杆菌O157:H7和沙门氏菌.
- 在所有隔离物中观察到细胞内胆汁盐酸酶活性,这表明潜在的降胆固醇效应.
结论:
- 引导式文化学工作流程有效地减少了细菌干扰,并为安全,功能性肠关联酵母提供了丰富的产品.
- 确定了几种有前途的非Candida酵母菌株,具有强大的益生菌潜力,包括显著的抗微生物和耐压能力.
- 这些发现支持开发基于酵母的新型益生菌来调节肠道微生物群并促进胃肠道健康.
相关概念视频
01:27Microbial Growth Media
1.2K
Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.
1.2K
01:24Techniques for Isolation of Pure Cultures
1.3K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
1.3K
01:20Overview Of Cell Separation And Isolation
7.0K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.0K
00:57Antibiotic Selection
59.3K
Overview
59.3K