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基于光检测基因组DNA中的KRAS突变,使用磁珠合LDR试验

Chika Morimoto1, Masahiko Hashimoto1

  • 1Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, 1-3 Tataramiyakodani, Kyotanabe 610-0321, Japan.

Methods and protocols
|December 24, 2025
PubMed
概括

一种新的珠联联酶检测反应 (LDR) 试验有效地检测结直肠癌细胞系中的单核酸变异 (SNV). 这种基于光的快速,经济高效的方法对遗传分析和诊断有很大的前景.

关键词:
光检测的检测方法酶检测反应 (LDR) 是一种磁性珠子 磁性珠子聚合酶连锁反应的发生.单核酸变异 (单核酸变异,简称SNV)

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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