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基因组流行病学研究方案密集护理病房患者被抗菌素耐药性ESKAPE病原体殖民的调查

Andrey Shelenkov¹, Oksana Ni², Irina Morozova³

¹Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia.

Methods and protocols

|December 24, 2025

概括

由于抗生素耐药性,ESKAPE细菌构成重大威胁. 这项研究详细介绍了一项全基因组测序协议,用于追踪在重症监护室 (ICU) 患者中抗微生物耐药 (AMR) 病原体的殖民化.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

逃生病原体是医疗保健相关感染的主要原因之一.
这些细菌迅速获得抗菌素耐药性 (AMR),需要进行严格的监测.
了解殖民动态对于预防重症监护室 (ICU) 中的严重感染至关重要.

研究的目的:

提出一个全面的协议,以表征ICU患者的AMR病原体定居的特征.
识别与耐药微生物群的获得相关的风险因素.
使用全基因组测序 (WGS) 分析抗生素耐药性的传播和机制.

主要方法:

从重症监护室患者收集直肠/耳鼻或呼吸道样本.
种植,物种识别和抗生素敏感性测试.
孤立的全基因组测序 (WGS) 用于确定耐药性基因,毒性因子和类型概况 (MLST,cgMLST).

主要成果:

描述物种分布,表型和基因型耐药性概况以及AMR病原体的克隆结构.
估计不同身体部位的检测频率和殖民模式.
确定与入院殖民相关的关键风险因素,包括先前的暴露.

结论:

该协议为在ICU中对AMR殖民的详细监测提供了一个框架.
WGS数据使病原体组成,耐药性趋势和克隆特征的全面分析成为可能.
这种方法有助于估计重症患者的AMR感染风险和严重程度.

关键词:

逃离病原体的可能抗微生物耐药性抗微生物耐药性细菌殖民化的细菌殖民重症监护病房是重症监护病房.整个基因组的测序.

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