由彩虹鱼线粒体产生的纳弗酸诱导的ROS排放
Zahra Kalvani1, Pius Tetteh1, Collins Kamunde1
1Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Toxics
|December 24, 2025
概括
废水中的纳芬酸分离化合物 (NAFC) 刺激了线粒体的活性氧物种 (ROS) 生产. 这项研究确定了线粒体复合体II (II_F) 作为鱼类中NAFC诱导的ROS的主要来源.
科学领域:
- 环境毒理学环境毒理学
- 线粒体生物化学 线粒体生物化学
- 水生毒理学 水生毒理学
背景情况:
- 纳弗酸分离化合物 (NAFC) 在石化废水中很常见.
- 在孤立的线粒体中,NAFCs可以诱导反应性氧物种 (ROS).
研究的目的:
- 为了研究3,5-二甲基亚胺-1-酸,NAFC,对线粒体ROS生产和消费的影响.
- 为了确定负责NAFC诱导ROS生成的特定线粒体位点.
主要方法:
- 隔离的彩虹鱼肝脏线粒体暴露于3,5-二甲基亚胺-1-酸.
- 在不同的呼吸状态下使用度测量测量了ROS排放量.
- 从特定的线粒体复合体中产生ROS的量化方法是使用Amplex UltraRed-马过氧化酶系统.
主要成果:
- 在线粒体复合体II (II_F) (EC50 = 0.2 mM) 中,3,5-二甲基亚胺-1-酸以剂量依赖的方式增加了ROS排放.
- 综合II (II_F) 被确定为NAFC刺激的ROS生产的主要地点.
- 马洛酸盐是一种糖酸脱酶的抑制剂,可以消除ROS的产生.
结论:
- 线粒体综合体II (II_F) 是由阿达曼坦型NAFCs诱导的ROS的主要来源.
- 在鱼类中,NAFCs通过刺激复合II的ROS产生来破坏线粒体的氧化还原平衡.
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