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通过PGC-1α/Nrf2信号通路对线粒体损伤的PEEK改性大气包装材料的食物迁移的影响

Sihui Guo¹, Kaile Li¹, Wei Li¹

¹Food Safety and Health Research Center, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.

|December 24, 2025

概括

食品包装中的聚乙乙 (PEEK) 通过破坏线粒体,可能会损害肝细胞. 这种线粒体毒性与PGC-1α/Nrf2通路有关,可以通过特定的激活剂减轻.

科学领域:

毒理学毒理学毒理学
生物化学生物化学
食品科学食品科学食品科学

背景情况:

聚乙乙 (PEEK) 用于修改大气层 (MAP) 包装,用于保存水果和蔬菜.
有关PEEK迁移到食品中的潜在健康风险存在担忧.

研究的目的:

研究PEEK食物迁移对肝细胞线粒体功能的影响.
为了阐明涉及PEEK诱导的线粒体损伤的分子通路.

主要方法:

使用食品模拟剂 (95%乙醇,10%乙醇,4%乙酸) 进行了迁移测试.
肝细胞被暴露在PEEK迁移产品中24小时.
评估了线粒体损伤标志物,包括细胞活力,活性氧物种 (ROS),线粒体膜潜力 (MMP) 和线粒体DNA拷贝数 (mtDNAcn).
分析了PGC-1α/Nrf2通路相关基因 (Sirt1,PGC-1α,NRF1,Nrf2,TFAM) 的表达.

主要成果:

PEEK迁移显著降低了肝细胞活力和线粒体膜潜力.
观察到活性氧物种 (ROS) 的水平增加和线粒体DNA拷贝数 (mtDNAcn) 的减少.
在PGC-1α/Nrf2通路 (Sirt1,PGC-1α,NRF1,Nrf2,TFAM) 中检测到关键基因的下调.
使用PGC-1α激活剂ZLN005的治疗逆转了这些有害影响,缓解了线粒体损伤.

结论:

高度的PEEK通过PGC-1α/Nrf2通路诱导肝细胞中的线粒体毒性.
这些发现突出了与食品包装中的PEEK相关的潜在健康风险.
确定PEEK在与食品接触材料中的安全剂量限制至关重要.

关键词:

皮克皮克 (Peek Peek) 是一种古老的风格.在PGC-1α/Nrf2中.线粒体损伤可能导致线粒体损伤.

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