与ChAdOx1-VZV的异质原始增强建立了双层免疫原性,赋予了对疹杆菌的保护潜力
Jiayu Zhao1, Juan Shao1, Xiuwen Sui1
1CanSino Biologics Research Center, Tianjin 300457, China.
Vaccines
|December 24, 2025
概括
一种新的ChAdOx1-VZV (CVE) 疫苗显示,它有望预防疹. 在临床前模型中,异质肌内主要/鼻内增强疗法显著增强了免疫反应,特别是CD8+T细胞活性.
科学领域:
- 免疫学和病毒学
- 疫苗开发 疫苗开发
- 传染性疾病 传染性疾病
背景情况:
- 疹病毒 (Varicella-zoster virus,简称VZV) 导致疹 (HZ),特别是在细胞介导免疫 (CMI) 衰退的老年人中.
- 目前的VZV疫苗在引起强大的CD8+T细胞反应方面存在局限性,这对于控制VZV重新激活至关重要.
- 开发了一种新的候选疫苗,ChAdOx1-VZV (CVE),利用黑猩猩腺病毒载体和糖蛋白E (gE) 抗原.
研究的目的:
- 为了评估新型ChAdOx1-VZV (CVE) 疫苗的免疫性.
- 为了评估CVE疫苗接种的异质肌内 (IM) 主/鼻内 (IN) 助推方案.
- 确定CVE作为预防疹病毒的候选疫苗的潜力.
主要方法:
- 通过使用各种同源和异源原始增强方案 (IM和IN) 用CVE免疫小鼠和Cynomolgus.
- 用细胞内细胞因子染色 (ICS) 和IFN-γ ELISpot测试来评估细胞免疫反应.
- 通过血清gE-IgG ELISA和骨髓LLPC ELISpot测定来测量幽默免疫反应.
主要成果:
- 不同类型的IM prime/IN增强疗法显著增强了细胞和幽默免疫反应,显著增加了CD8+T细胞活性.
- 通过诱导长寿血细胞 (LLPC) 和记忆T细胞反应,建议提供长期保护.
- 在Cynomolgus中,CVE表现出强大的免疫性,显示出强烈的gE特异性IgG和IFN-γT细胞反应,支持其临床翻译潜力.
结论:
- ChAdOx1-VZV (CVE) 疫苗,特别是IM prime/IN提升策略,可以诱导强大的细胞和幽默免疫反应.
- 非人类灵长类动物的跨物种免疫性验证了CVE平台的翻译相关性.
- CVE是疹疫苗的有希望的候选者,需要在人体系统中进一步评估.
更多相关视频
08:04Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
Published on: July 9, 2014
16.2K
08:10Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
Published on: November 28, 2018
9.0K
相关概念视频
Development of Immunocompetence
769
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
769
Immunological Memory
14.9K
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
14.9K
Immune Response Against Viral Pathogens
1.6K
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
1.6K
Antigens Involved in Adaptive Immunity
1.2K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
1.2K
Vaccinations
51.1K
Overview
51.1K
Humoral Immune Responses
83.2K
Overview
83.2K
