Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Photoluminescence: Applications01:14

Photoluminescence: Applications

965
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
965
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

1.2K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.2K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

28.0K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
28.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Phenoxazines with a Phototransferable <i>N</i>-Acetyl Group and Acrylate Linker: Assembly by C-H Activation, Photoconversion to Fluorescent Dyes, Biolabeling, and Super-Resolution Imaging.

Journal of the American Chemical Society·2026
Same author

Synthesis of Benzo[<i>b</i>]thiophene 1,1-Dioxides via Pd-Catalyzed Sulfinylation of Aryl Triflates and Their Use as Large Stokes Shift Fluorophores for Multicolor Live-Cell Imaging with Self-Labeling Tags.

JACS Au·2026
Same author

3D-Helical Artificial Chromatophore for Thermally Tunable Fluorescence and Stealth Switching.

ACS applied materials & interfaces·2026
Same author

Photoactivatable Carborhodol and Carborhodamine Dyes with One Cleavable Group: Synthesis, Spectra, and Fluorescence Nanoscopy Applications.

JACS Au·2026
Same author

Arc-discharge-assembled CNT/MoO<sub>3</sub> nanohybrids for ultra-sensitive and selective sub-ppm ethanol detection at room temperature.

RSC advances·2026

相关实验视频

Updated: Jan 7, 2026

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

17.6K

具有极点群的光日利烯:合成,光谱和光学显微镜应用.

Kakishi Uno1, Sven Nagorny1, Ayse Aktalay2

  • 1Department of NanoBiophotonics, Max Planck Institute For Multidisciplinary Sciences (MPINAT), Göttingen, Germany.

Chemistry (Weinheim an der Bergstrasse, Germany)
|December 24, 2025
PubMed
概括

研究人员开发了用于先进显微镜的新型可光激活光二乙烯 (fDAE). 这些探测器使细胞结构的多色超分辨率成像能够具有改进的光谱特性.

关键词:
染色体是染色体的组成部分每日记里面写着一些事情.光是一种光.摄影化学 摄影化学这是光色染色主义.

更多相关视频

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.6K
Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core
08:51

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core

Published on: October 24, 2017

10.0K

相关实验视频

Last Updated: Jan 7, 2026

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
12:07

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes

Published on: April 1, 2013

17.6K
Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
14:11

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Published on: June 10, 2021

6.6K
Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core
08:51

Scale-up Chemical Synthesis of Thermally-activated Delayed Fluorescence Emitters Based on the Dibenzothiophene-S,S-Dioxide Core

Published on: October 24, 2017

10.0K

科学领域:

  • 化学生物学 化学生物学
  • 光学显微镜的使用方法
  • 超分子化学 超分子化学

背景情况:

  • 可光激活光二乙烯 (fDAE) 为生物成像提供可调节的光谱特性.
  • 目前的fDAE存在结构变化和光谱控制方面的局限性.
  • 开发新的fDAE对于推进光显微镜技术至关重要.

研究的目的:

  • 合成具有可适应的结构和光谱特征的新型"启动"fDAE.
  • 为了创建细胞透的线粒体选择性探针和蛋白质生物结合物.
  • 用新开发的fDAE来演示多色超分辨率成像.

主要方法:

  • 合成具有四个基团和单个核心功能的HO-fDAE.
  • 细胞透性线粒体选择性探针和蛋白质生物结合物的制备.
  • 在多色成像中,HO-fDAE与硫替代的fDAE (Th-fDAE) 的组合.

主要成果:

  • HO-fDAE发出绿灯,并使生物结合成为可能.
  • Th-fDAE表现出红移吸收/排放和高效的开关.
  • 较大的斯托克斯移位 (80-110 nm) 允许分离颜色和双色超分辨率成像.
  • 对HO-fDAE的光激活和Th-fDAE的闪使多色成像成为可能.

结论:

  • 新的HO-fDAE和Th-fDAE克服了基于fDAE的显微镜的局限性.
  • 开发的探测器有助于细胞结构的多色超分辨率成像.
  • 这些fDAE在化学生物学和先进成像学中提供了多方面的应用.