m6 修改和SUMOylation的PHGDH调节性结肠炎通过自
Hui Xue1, Dandan Wang2, Chengjian Pang2
1Department of Gynecology, Qingdao Hospital of Traditional Chinese Medicine, Qingdao, Shandong Province, 266000, China.
Cellular and molecular life sciences : CMLS
|December 24, 2025
概括
糖酸脱酶 (PHGDH) m6A修饰和SUMOylation通过影响自来调节性结肠炎 (UC). 用黄素等化合物准METTL3/UBC9/PHGDH轴可能可以治疗UC.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,全球发病率正在上升.
- 目前尚不完全了解UC病原发生的精确机制.
研究的目的:
- 调查糖酸脱酶 (PHGDH) 在性结肠炎 (UC) 中的作用.
- 阐明涉及m6A修饰和UC中PHGDH的SUMOylation的调节机制.
- 探索针对METTL3/UBC9/PHGDH轴的潜在治疗效果.
主要方法:
- 已建立的DSS诱导的UC小鼠和LPS治疗的结肠上皮细胞 (CEC) 模型.
- 评估病理变化,基因/蛋白质表达和分子修饰 (m6A,SUMOylation) 使用诸如qRT-PCR,西部斑点,ELISA和MeRIP测定等技术.
- 通过CCK-8测定和共免疫沉评估细胞活力和蛋白质稳定性.
主要成果:
- 通过促进自,PHGDH的淘汰和抑制减轻了结肠炎症状和炎症.
- 甲基转移酶样3 (METTL3) 介导PHGDH的m6A修饰,而UBC9介导的SUMOylation调节了PHGDH的稳定性.
- 通过调节UBC9/PHGDH轴,METTL3抑制了自;METTL3敲击和UBC9过度表达协同减少了结肠损伤.
- 黄素治疗通过METTL3/UBC9/PHGDH轴促进了自,减轻了结肠损伤.
结论:
- PHGDH的m6A修饰和SUMOylation是通过Beclin-1-依赖自的UC病变发生的关键调节者.
- METTL3/UBC9/PHGDH轴代表了UC的潜在治疗目标.
- 通过这个轴调节自为UC治疗提供了一个有前途的策略.
相关概念视频
Autophagy
5.6K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.6K
Covalently Linked Protein Regulators
8.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
8.6K
Abnormal Proliferation
5.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Receptor Downregulation in MVBs
2.7K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.7K
mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
Delivery Pathways to the Lysosome
8.7K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.7K


