Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An E3 Aptamer-Modified T Cell-Derived Exosomal Nanoplatform for Codelivery of Astragaloside IV and PESV: Enhancing Prostate Cancer Therapy Through Immune Modulation with Implications for Clinical Translation.

International journal of nanomedicine·2026
Same author

Superior Hydrodesulfurization Catalyst Prepared via Hydrothermal Deposition of Lacunary NiPW Polyoxometalate on γ-Al<sub>2</sub>O<sub>3</sub>.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Suppression of LncRNA AC008406.3 sensitizes breast cancer cells to docetaxel via triggering cuproptosis.

Cancer biology & therapy·2026
Same author

The CPR compression matrix: relationship among depth, recoil, and rate.

Resuscitation·2026
Same author

Manual synchrony may not equal electrical synchrony in double defibrillation.

Resuscitation·2026
Same author

Synthesis and Applications of the Se-N Clickable and Orthogonal Linker Based on Bicyclo[1.1.1]pentane.

Organic letters·2026

相关实验视频

Updated: Jan 7, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.2K

通过并行像素重新分配的增强分辨率线扫描共聚焦显微镜.

Yanchi Li, Jian Liu, Sida Chen

    Optics letters
    |December 24, 2025
    PubMed
    概括

    本研究介绍了使用数字微镜装置 (DMD) 的直角多线像素重新分配 (OML-PR),以提高共聚焦显微镜的速度和分辨率. 通过OML-PR方法,可以实现无文物成像和快速3D扫描,用于微纳米结构分析.

    科学领域:

    • 光学显微镜的使用方法
    • 纳米技术纳米技术
    • 图像处理 图像处理

    背景情况:

    • 同焦显微镜面临着分辨率速度的权衡.
    • 数字微镜设备 (DMD) 提供先进的照明控制.

    研究的目的:

    • 为了提高共聚焦显微镜中的分辨率和成像速度.
    • 开发一种方法来实现无人工物同otropic分辨率增强.
    • 为了提高微纳米结构地形检查的效率.

    主要方法:

    • 使用DMD进行直角多行像素重新分配 (OML-PR).
    • 高密度直角多线结构照明,用于并行横扫描.
    • 三维协调连续轴向扫描.
    • 梯度引导的重新分配机制用于无工件的分辨率增强.

    主要成果:

    • 在455nm波长下实现了137nm横向分辨率与100×/0.90 NA目标.
    • 成功检测到98nm间隙缺陷和48nm桥梁缺陷.
    • 在不到3秒的时间内完成完整的3D扫描.

    结论:

    更多相关视频

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.5K
    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
    10:41

    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

    Published on: May 19, 2022

    2.5K

    相关实验视频

    Last Updated: Jan 7, 2026

    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
    09:20

    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

    Published on: August 11, 2020

    7.2K
    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
    10:07

    Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

    Published on: April 9, 2014

    10.5K
    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
    10:41

    Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

    Published on: May 19, 2022

    2.5K
    • 在共聚焦显微镜中,OML-PR方法显著提高了采集效率和分辨率.
    • 该技术使得微纳米结构的高质量重建和高效检查成为可能.
    • 证明了一种可行的方法来克服先进成像中的分辨率速度限制.