通过JAK2 / STAT3介导的干细胞激活,保护针提取物免受沙门氏菌引起的 brojlers肠道功能障碍
Yuqing Feng1, Yaxue Lv1, Hang Chen1
1Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Animal Nutrition Control, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
爪提取物通过抑制细菌生长和改善肠道健康,有效地对抗 brojler 中的 Salmonella Enteritidis (SE). 这种天然抗菌剂可以保护肠道组织,并在无抗生素生产系统中增强的生长.
科学领域:
- 禽畜科学 禽畜科学 禽畜科学
- 微生物学 微生物学
- 动物营养 动物营养
背景情况:
- 在没有抗生素的肉中,沙门氏菌 (Salmonella Enteritidis,SE) 感染会导致显著的肠损伤.
- 丁香的提取物是一种天然的抗菌剂,有可能减轻SE引起的肠道损伤.
- 爪提取物对SE进行保护的确切机制在很大程度上仍未被阐明.
研究的目的:
- 为了确定南瓜提取物对肉沙门氏菌 (SE) 的疗效.
- 阐明南瓜提取物对SE引起的肠损伤的保护作用的潜在机制.
- 评估针叶提取物作为无抗生素家禽生产的植物治疗候选物.
主要方法:
- 确定植物提取物针对SE的最小抑制度 (MIC) 和抑制区.
- 在肉中建立了SE诱导的肠损伤模型.
- 评估了肠道屏障功能 (血清LPS,DAO,TEER),肠道形态,干细胞动态 (Lgr5,Olfm4,SOX9,PCNA) 和JAK2/STAT3通路的激活.
主要成果:
- 爪提取物表现出强大的抗菌活性对SE,具有最低的MIC和最大的抑制区.
- SE感染显著损害了肠道屏障功能,减少了肠道重量,小高度和小/密室比率.
- 爪子提取物 (300毫克/公斤) 逆转了SE诱导的损伤,降低了血清DAO和LPS,增加了阴茎重量,高度,TEER和升调的屏障蛋白 (Occludin).
- 爪提取物促进了肠干细胞 (ISC) 的增殖和分化,增强了有机体的形成,并抑制了过度的JAK2/STAT3通路激活.
结论:
- 爪提取物对SE具有显著的抗菌作用,并减轻SE诱导的JAK2/STAT3通路过活化.
- 爪提取物增强肠道干细胞的增殖和分化,具有双重抗菌和抗炎作用.
- 爪提取物是一种有前途的植物治疗剂,用于改善无抗生素家禽生产中SE诱导的肠损伤.
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